Activation and steady-state inactivation characteristics of Cav2.3 channels in Cav2.3/μ-, δ-, or κ-OR cells
Parameters derived from G−V and steady-state inactivation curves fitted with the Boltzmann equation. In all cases, the concentration of the OR agonist and OR antagonist was 1 μM and 100 nM, respectively. Values represent the mean ± S.E.M. (n, number of experiments, in parentheses).
| Condition (n) | V0.5, act | k | V0.5, inact | k |
|---|---|---|---|---|
| mV | mV | mV | mV | |
| Cav2.3/μ-OR | ||||
| Control (8) | 1.2 ± 1.4 | 4.8 ± 0.3 | −56.8 ± 1.7 | 9.6 ± 0.5 |
| DAMGO (8) | 1.6 ± 1.4 | 5.4 ± 0.2 | −57.1 ± 1.3 | 9.9 ± 0.6 |
| Naloxone (5) | 0.5 ± 2.1 | 4.4 ± 0.9 | −56.9 ± 1.4 | 11.1 ± 0.7 |
| Cav2.3/δ-OR | ||||
| Control (8) | 3.7 ± 1.2 | 5.5 ± 0.3 | −54.3 ± 1.6 | 9.6 ± 0.5 |
| SNC80 (8) | 3.3 ± 1.0 | 5.7 ± 0.3 | −54.5 ± 1.5 | 9.9 ± 0.8 |
| BNTX (6) | 2.2 ± 1.4 | 5.2 ± 0.9 | −55.4 ± 1.9 | 10.9 ± 0.9 |
| Cav2.3/κ-OR | ||||
| Control (7) | 2.9 ± 1.5 | 5.4 ± 0.1 | −56.7 ± 2.1 | 9.1 ± 0.6 |
| Sal-A (7) | 3.5 ± 1.6 | 5.5 ± 0.1 | −57.0 ± 2.8 | 9.6 ± 0.5 |
| GNTI (4) | 2.6 ± 2.0 | 4.9 ± 0.8 | −59.9 ± 1.8 | 11.4 ± 1.5 |