Adenylyl cyclase inhibition, G-protein activation and β arrestin-2 recruitment induced by norepinephrine mediated by α2AR
Data of individual experiments were fit by nonlinear regression to a sigmoidal dose–response relationship against the agonist concentration. The EC50 and Emax values are the mean ± S.E.M. of 3 to 4 experiments performed in triplicate. Emax values are expressed as 1000 × [BRETmax − BRETbasal].