Potency (pEC50) and relative efficacy (Emax) of TUG-891 at the mFFA4-WT and mutant receptors in pERK1/2 and pAkt (Ser473) assays
Values represent the mean ± S.E.M. from three independent experiments performed in triplicate.
| Receptor | pERK 1/2 | pAkt (Ser473) | ||||
|---|---|---|---|---|---|---|
| pEC50 | Emax | n | pEC50 | Emax | n | |
| mFFA4-WT | 7.8 ± 0.2 | 100 | 3 | 8.0 ± 0.2 | 100 | 3 |
| mFFA4-ADAASSS | 8.1 ± 0.1 | 106 ± 2 | 3 | 8.4 ± 0.1 | 76 ± 1 | 3 |
| mFFA4-TDTSAAA | 8.0 ± 0.2 | 111 ± 1 | 3 | 8.0 ± 0.1 | 99 ± 1 | 3 |
| mFFA4-ADAAAAA | 8.2 ± 0.2 | 116 ± 2 | 3 | 8.4 ± 0.4 | 63 ± 2 | 3 |