Sequences of primers used for amplifying GLI1 DNA fragments
T7 RNA promoter sequences are underlined.
| Name | Sequences (5′ to 3′) |
|---|---|
| GLI1 36–231 | Forward: GGATCCTAATACGACTCACTATAGGGCCATGTTCAACTCGATG |
| Reverse: CCTCGGTGCAGCTGTTGG | |
| GLI1 230–420 | Forward: GGATCCTAATACGACTCACTATAGGGGGCCCACTCTTTTCTTC |
| Reverse: TGCCAATGGAGAGATGAC | |
| GLI1 420–610 | Forward: GGATCCTAATACGACTCACTATAGGACCATGAGCCCATCTCTG |
| Reverse: CGGCACTTGCCAACCAGC | |
| GLI1 610–800 | Forward: GGATCCTAATACGACTCACTATAGGGGGAGGAACCCTTGGAAG |
| Reverse: GTGCACCAGCTGCTCTTG | |
| GLI1 800–990 | Forward: GGATCCTAATACGACTCACTATAGGCCACATCAACAGCGAGCA |
| Reverse: GGTTTTCGAGGCGTGAGT | |
| GLI1 230–380 | Forward: As above for GLI1 230-420 |
| Reverse: GCATCGCGAGTTGATGAA | |
| GLI1 230–330 | Forward: As above for GLI1 230-420 |
| Reverse: CCGTCTGCAGGTCCAGGC | |
| GLI1 230–300 | Forward: As above for GLI1 230-420 |
| Reverse: GAGGTGAGATGGACAGTG | |
| GLI1 230–275 | Forward: As above for GLI1 230-420 |
| Reverse: CTTGGTCAACTTGACTGC | |
| GLI1 270–420 | Forward: GGATCCTAATACGACTCACTATAGGACCAAGAAGCGGGCACTG |
| Reverse: As above for GLI1 230-420 | |
| GLI1 320–420 | Forward: GGATCCTAATACGACTCACTATAGGCCTGCAGACGGTTATCCG |
| Reverse: As above for GLI1 230-420 | |
| GLI1 350–420 | Forward: GGATCCTAATACGACTCACTATAGGCTCCCTCGTAGCTTTCAT |
| Reverse: As above for GLI1 230-420 | |
| GLI1 375–420 | Forward: GGATCCTAATACGACTCACTATAGGCGATGCACATCTCCAGGA |
| Reverse: As above for GLI1 230-420 | |
| GLI1 320–380 | Forward: As above for GLI1 320-420 |
| Reverse: As above for GLI1 230-380 |