TABLE 3

Effect of geldanamycin on current facilitation evoked by 60-second application of ATP in wild-type P2X7R and its mutants

Values are mean ± S.E.M.; n, number of experiments.

ChimeraI1(pA/pF)I2(pA/pF)I1/IcontrolI2/Icontroln
X7-X7-X7−110 ± 24−239 ± 430.9 ± 0.02.4 ± 0.414
+ geldanamycin−198 ± 45−253 ± 652.1 ± 0.5*2.5 ± 0.411
X2-X7-X7−474 ± 194−650 ± 2320.7 ± 0.11.0 ± 0.15
+ geldanamycin−427 ± 121−557 ± 1171.0 ± 0.11.4 ± 0.25
X7-X7-X2−2 ± 1−1 ± 10.8 ± 0.40.4 ± 0.23
+ geldanamycin−5 ± 2−4 ± 11.1 ± 0.00.7 ± 0.13
X2-X7-X2−74 ± 34−18 ± 80.5 ± 0.20.1 ± 0.06
+ geldanamycin−273 ± 108−56 ± 170.5 ± 0.10.1 ± 0.03
X7[Δ18]−101 ± 25−78 ± 260.8 ± 0.10.6 ± 0.18
+ geldanamycin−278 ± 89−188 ± 660.7 ± 0.10.5 ± 0.14
  • * P < 0.05, **P < 0.01 versus Normal solution (Student’s t test).