TABLE 2

Permeability of the wild-type P2X7R and its mutants to NMDG⁺

Reversal potential (E_rev) and relative permeability (P_NMDG/P_Cs) for wild-type P2X7R and its chimeras at the beginning (1 second) and end (60 seconds) of 60-secons application of ATP. Values are mean ± S.E.M.

Chimera	E_rev		Δv_rev	P_NMDG/P_Cs		n
Chimera	1 sec	60 sec	Δv_rev	1 sec	60 sec	n
	mV	mV	mV
X7-X7-X7	−38.9 ± 1.7	−13.0 ± 5.9^b	25.9 ± 5.7	0.21 ± 0.01	0.67 ± 0.12^{^b}	6
+ geldanamycin	−39.4 ± 3.0	−31.9 ± 2.7^{^a}^′	7.7 ± 1.2^{^a}	0.22 ± 0.03	0.29 ± 0.04^a	7
X2-X7-X7	−45.4 ± 1.1	−35.9 ± 1,4^ab	9.5 ± 1.7^{^a}	0.17 ± 0.01	0.24 ± 0.01^{^a}	5
+ geldanamycin	−39.1 ± 0.6	−34.9 ± 2.1^{^a}	4.3 ± 2.0^{^a}	0.21 ± 0.01	0.26 ± 0.02^{^a}	7
X7-X7-X2	−41.8 ± 7.3	−39.4 ± 6.6^{^a}	2.4 ± 2.5^{^a}	0.23 ± 0.07	0.25 ± 0.06^{^a}	6
+ geldanamycin	−42.5 ± 2.0	−43.2 ± 2.9^{^a}	2.6 ± 2.0^{^a}	0.19 ± 0.02	0.19 ± 0.02^{^a}	5
X2-X7-X2	−46.6 ± 3.9	−39.5 ± 6.5^{^a}	8.8 ± 2.3^{^a}	0.16 ± 0.02	0.24 ± 0.05^{^a}	6
+ geldanamycin	−37.6 ± 2.0	−37.4 ± 3.5^{^a}	1.3 ± 1.7^{^a}	0.23 ± 0.02	0.24 ± 0.03^{^a}	5
X7[A18]	−33.5 ± 2.0	−33.5 ± 1,0^{^a}^′	2.1 ± 0.9^{^a}	0.27 ± 0.02	0.27 ± 0.01^{^a}	6
+ geldanamycin	−31.5 ± 1.5	−35.1 ± 3.4^{^a}	−3.6 ± 2.6^{^a}	0.29 ± 0.02	0.26 ± 0.04^{^a}	5
X2-X2-X2	−47.9 ± 1.0	−32.6 ± 2.4^{^a}^′^{^b}	15.8 ± 5.3	0.15 ± 0.01	0.28 ± 0.03^ab	6

n, number of experiments.
↵^a ^′P < 0.05, ^aP < 0.01 (Tukey’s test), significant differences between wild-type P2X7R and others.
↵^b ^′P < 0.05, ^bP < 0.01 (Student’s t test), significant shifts between 1 second and 60 seconds.