Sequence of primers used for quantitative reverse-transcription PCR
| Isoforms and Primers | Sequence |
|---|---|
| CPH | |
| CPH-S | 5′-CAG ACG CCA CTG TCG CTT T-3′ |
| CPH-AS | 5′-TGT CTT TGG AAC TTT GTC TGC AA-3′ |
| UGT1A1 | |
| UGT1A1-S | 5′-CCT TGC CTC AGA ATT CCT TC-3′ |
| UGT1A1-AS | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ |
| Sult1b1 | |
| Sult1b1-S | 5′-TCC ATC TCA GGT CAC CAC CA-3′ |
| Sult1b1-AS | 5′-TCT CCA AAC GTC TTC TGA GGC-3′ |
| D1 | |
| D1-S | 5′-GTT TAG CAC AAG CAA GAG GCA-3′ |
| D1-AS | 5′-GCG CCT GCG ATT TGG TTT AG-3′ |
| D2 | |
| D2-S | 5′-GAA TCC CAT TGC CTC ACC GA-3′ |
| D2-AS | 5′-AGG CTG CAA CAG GGT TTC TT-3′ |
| D3 | |
| D3-S | 5′-ATT GCT GTG GCT CGA ACT GA-3′ |
| D3-AS | 5′-GAA ATG CTG GGG ACT TTC GC-3′ |
| Synapsin I | |
| Synapsin I-S | 5′-TGC CAA CAA GAC GGA GAG TG-3′ |
| Synapsin I-AS | 5′-TAG TGC CCC CTT TAA CGC AG -3′ |
| Synaptophysin | |
| Synaptophysin-S | 5′-TTT GCC ATC TTC GCC TTT GC-3′ |
| Synaptophysin-AS | 5′-GTG CAG CCT GAA TGG GTA CT-3′ |
AS, antisense; CPH, cyclophilin; D1, type 1 iodothyronine deiodinase; D2, type 2 iodothyronine deiodinase; D3, type 3 iodothyronine deiodinase; S, sense.