Kinetic rate and equilibrium binding constant values which were determined by SPR for the interaction of hRS1-Reg(S20A) and hRS1-Reg(S20E) with immobilized hODC1
SPR analysis was performed in the absence and presence of 1 mM d-glucose or after treatment of the flow channel with 1 mM DFMO. Mean values ± S.D. from three independent experiments are shown. The differences between hRS1-Reg(S20A) and hRS1-Reg(S20E), hRS1-Reg(S20E) and hRS1-Reg(S20E) with 1 mM d-glucose, and hRS1-Reg(S20E) and hRS1-Reg(S20E) with 1 mM DFMO are not statistically significant.
| kon [×104 M−1s −1] | koff [×10−3 s−1] | KD[nM] | |
|---|---|---|---|
| hRS1-Reg(S20A) | 0.9 ± 0.2 | 0.6 ± 0.1 | 68 ± 20 |
| hRS1-Reg(S20E) | 0.8 ± 0.3 | 0.9 ± 0.4 | 102 ± 25 |
| hRS1-Reg(S20E) with 1 mM d-glucose | 0.5 ± 0.1 | 0.7 ± 0.1 | 161 ± 35 |
| hRS1-Reg(S20A) with 1 mM DFMO | 0.9 ± 0.7 | 0.6 ± 0.4 | 85 ± 12 |