TABLE 1

Primers used to prepare reporter constructs and competitor oligonucleotides used for PPARγ in vitro binding assay

NameSequence
Primers for reporters
 SULT1C3-TSS reversea5′-GGG CTC GAG GCT CCA GGA CAC TGT GCA AGC AA-3′
 SULT1C3-2.8Kb forward5′-GGG GGT ACC TCT GGT CCT CCT TCA TTC CCG CAA-3′
 SULT1C3-1Kb forward5′-GGG GGT ACC ATG CTC TAC ATA ATT CAC GTC-3′
 SULT1C3-DEL1 forward5′-GGG GGT ACC ACA GAG GAC AGA CAA TGT AAA T-3′
 SULT1C3-DEL2 forward5′-GGG GGT ACC TTT TAT TAC AGG CCT TGT GGT-3′
 SULT1C3-DEL3 forward5′-GGG GGT ACC TTT CTA CAG GGT CAA AGG GA-3′
 SULT1C3-DEL4 forward5′-GGG GGT ACC AAC AGG ATG AAA TAA TTG TGC-3′
 SULT1C3-MUT1 senseb5′-GGA GTT AAG TAA ATA TTG TAC AGA AGG TAT TGT TAA AAT TCC ATA TAT TTA CAT TGTT CTG TCC TCT GTT TTG CAA-3′
 SULT1C3-MUT2 sense5′-CCG TAG TTA AAA TTG GTG TAG AAG AAA AAG CTT TTT AGG AAA CCA CAA GGC CTGT TAA AAC-3′
 SULT1C3-MUT3 sense5′-ACT TGC ACA ATT ATT TCA TCC TGT TCC CTG GAT CCC TGT AGA AAA TAT ATT CTA TTG CCT CT-3′
Competitor oligonucleotides for PPARγ in vitro binding assay
 SULT1C3-WT PPRE senseb5′- AAC AAT GAA CTC TGT ACA ATA TTT -3′
 SULT1C3-MUT PPRE sense5′- AAC AAT ACC TTC TGT ACA ATA TTT -3′
 CYP4A1-WT PPRE sense5′- GAA ACT AGG GTA AAG TTC AGT GAG -3′
 CYP4A1-MUT PPRE sense5′- GAA ACT CGG AGC ACG TTA AGT GAG -3′

  • ↵a The same reverse primer was used to prepare the 2.8-kb, 1.9-kb, 1-kb, DEL1, DEL2, DEL3, and DEL4 SULT1C3 fragments.

  • ↵b Only sense-strand sequences are shown for site-directed mutagenesis primers and in vitro binding assay oligonucleotides.