WT | H17A/H19A | |||
---|---|---|---|---|
ZnCl2 (+) (N = 8) | ZnCl2 (−) (N = 8) | ZnCl2 (+) (N = 5) | ZnCl2 (−) (N = 5) | |
GPR39-C3 | ||||
Top | 30.27 ± 0.57 | 33.25 ± 2.31 | 25.87 ± 0.55 | 29.07 ± 2.02 |
Bottom | 1.09 ± 0.36 | 1.00 ± 0.16 | 1.08 ± 0.34 | 1.00 ± 0.24 |
Log EC50 | −7.99 ± 0.04 | −6.22 ± 0.11 | −7.76 ± 0.04 | −6.43 ± 0.10 |
Hill slope | 1.52 ± 0.17 | 0.69 ± 0.05 | 1.51 ± 0.18 | 0.84 ± 0.10 |
LY2784544 | ||||
Top | 23.16 ± 0.52 | 7.10 ± 0.78 | 19.26 ± 0.56 | 7.23 ± 0.97 |
Bottom | 0.89 ± 0.25 | 1.00 ± 0.03 | 0.91 ± 0.27 | 1.00 ± 0.06 |
Log EC50 | −8.17 ± 0.04 | −5.81 ± 0.11 | −8.07 ± 0.04 | −5.91 ± 0.12 |
Hill slope | 1.45 ± 0.15 | 1.27 ± 0.16 | 1.61 ± 0.23 | 1.57 ± 0.38 |
GSK2636771 | ||||
Top | 22.61 ± 0.59 | N.D. | 22.85 ± 0.93 | N.D. |
Bottom | 0.831 ± 0.21 | 1.00 ± 0.03 | 0.91 ± 0.27 | 1.00 ± 0.07 |
Log EC50 | −7.068 ± 0.04 | >−4.00 | −6.94 ± 0.06 | >−4.00 |
Hill slope | 1.179 ± 0.11 | 1.59 ± 0.86 | 1.05 ± 0.13 | 1.703 ± 0.75 |
N.D., could not determine; WT, wild type.