TABLE 2

The response to physostigmine is less when the α2 subunit replaces a single α4 subunit

The first column gives the subunit combination injected. The concatemer construct α4-β2 has the α4 subunit at the amino-terminal end joined to the β2 subunit by a linker, whereas the β2-α4 concatemer has the β2 subunit at the amino-terminal end. The concatemers assemble in a clockwise fashion (N-terminal to C-terminal) as viewed from the extracellular space (Zhou et al., 2003; Jin and Steinbach, 2011). The column headed “P to α2” gives the P value that the parameter (EC₅₀ or effect ratio) is the same for the concatemer expressed with the studied subunit as with the α2 subunit (two-tailed t test with unequal variances).