RVX-297 and FTY720 reduce inflammation, demyelination, and apoptosis in the spinal cords of EAE mice
Histology was assessed in the cervical, thoracic, and lumbar regions of the spinal cords of EAE mice. Each parameter is presented as an average from these regions compared with vehicle-treated disease controls. Student’s t test (P values included in table) was used to determine statistical significance compared with vehicle-treated EAE mice [n = 10 mice/group (except for naive mice, n = 3)]. Data are the mean ± S.D.
| Group Treatment | Inflammatory Foci | P value | Demyelination (LFB) | P value | Apoptotic Cells | P value |
|---|---|---|---|---|---|---|
| Preventative treatment regimen | ||||||
| Naive | 0.0 ± 0.0 | <0.0001 | 0.0 ± 0.0 | <0.0001 | 0.0 ± 0.0 | <0.0001 |
| Vehicle | 4.3 ± 3.2 | NA | 1.6 ± 1.0 | NA | 2.7 ± 2.5 | NA |
| FTY720 (3 mg/kg) | 0.3 ± 1.0 | <0.0001 | 0.0 ± 0.2 | <0.0001 | 0.1 ± 0.4 | <0.0001 |
| RVX-297 (75 mg/kg) | 1.5 ± 2.9 | 0.0004 | 0.1 ± 0.3 | <0.0001 | 0.5 ± 0.9 | <0.0001 |
| RVX-297 (125 mg/kg) | 0.1 ± 0.6 | <0.0001 | 0.0 ± 0.0 | <0.0001 | 0.0 ± 0.0 | <0.0001 |
| Therapeutic treatment regimen | ||||||
| Naive | 0.0 ± 0.0 | <0.0001 | 0.0 ± 0.0 | <0.0001 | 0.0 ± 0.0 | <0.0001 |
| Vehicle | 3.9 ± 2.4 | NA | 1.6 ± 0.8 | NA | 2.0 ± 1.8 | NA |
| FTY720 (3 mg/kg) | 0.8 ± 0.9 | <0.0001 | 0.8 ± 0.5 | <0.0001 | 0.8 ± 0.9 | 0.002 |
| RVX-297 (75 mg/kg) | 1.7 ± 1.2 | <0.0001 | 1.1 ± 0.8 | 0.02 | 0.5 ± 1.0 | 0.0002 |
| RVX-297 (125 mg/kg) | 1.6 ± 1.9 | 0.0001 | 0.9 ± 0.8 | 0.002 | 0.3 ± 0.6 | <0.0001 |
LFB, Luxol fast blue; NA, not applicable.