TABLE 3 

Inhibition of Cys-Null RGS: Gαo interaction by RGS inhibitors

Biochemical characterization of RGS inhibitors for their ability to disrupt the cys-null RGS: Gαο PPI using AlphaScreen assay. Data represent the IC50 with the 95% CI in parentheses from n = 3 independent experiments, each with duplicate wells. Concentration-response curves are shown in Supplemental Fig. S2. All compounds from Table 2 were tested, and any compounds for which all cys-null RGS proteins exhibited >100 μΜ IC50 values were excluded from this table.

RGSIC50, μM (95% CI)
CCG-55919CCG-50014
1>100>100
4>100>100
5>100>100
8>100>100
10>100>100
1494 (60 to >100)40 (22–92)
16>100>100
17>100>100
18>100>100