TABLE 2 

Potencies and efficacies of CP55940 and LY2828360 in internalization, cyclase, and pERK1/2 assays at human CB2 receptors

Duration of drug incubation is expressed in minutes. cAMP accumulation assays were performed in duplicate. All other assays were performed in triplicate. EC50, 95% confidence intervals (CI), and the maximal effect (Emax, mean ± S.E.M.) were obtained by plotting and analyzing the data using GraphPad Prism 4.

CP55940LY2828360
Drug IncubationEC5095% CIEmax± S.E.M.EC5095% CIEmax±S.E.M.
Internalizationmin
90nM
30.3–15.6%
33.9±4.6nM
NDND%
10.2±7.1
Cyclase0512.32.9–18.359.6±8.3NDNDNDND
3516.74.6–59.642.8±2.7
pERK1/2053.770.4–12.795.7±9.1NDND22.1±5.8
3023.310.1–53.949.4±1.633.59.1–107.132.3±1.9
  • ND, Not determined or cannot be determined.