TABLE 1 

Potencies and efficacies of CP55940 and LY2828360 in arrestin, internalization, cyclase, and pERK1/2 assays at mouse CB2 receptors

Duration of drug incubation is expressed in minutes. All assays were performed in triplicates except cAMP accumulation assays, which were performed in duplicate. EC50, 95% confidence intervals (CI), and the maximal effect (Emax) (mean ± S.E.M.) were obtained by plotting and analyzing the data using GraphPad Prism 4.

CP55940LY2828360
Drug Incubation EC5095% CIEmax ± S.E.M.EC50 95% CIEmax (%)±S.E.M.
Arrestinmin
90nM
2.30.4–12.2%
125±1.6nM
NDND%
97.9±1.5
internalization907.41.1–19.349.1±1.230.71.4–626.519.1±2.4
Cyclase056.61.7–12.252.8±3.6NDND18.9±5.8
3013.610.4–45.353.4±1.9
pERK1/20510.52.2–17.9136.2±4.1NDND4.1±2.5
201.50.1–3.720.3±3.4339128.8–345.843.6±2.3
  • ND, Not determined or cannot be determined.