TABLE 2

Concentration-response data for glutamate and glycine

Data were measured using two-electrode voltage-clamp recordings of responses from NMDA receptors expressed in Xenopus oocytes. Glutamate concentration-response data were determined in the continous presence of 100 µM glycine, and glycine concentration-response data were determined in the continous presence of 100 µM glutamate. Data are presented as mean ± S.E.M., and n is the number of oocytes used to generate the data. Statistical tests were performed using log EC₅₀ values, and GluN2A-containing receptors were not compared with GluN2B-containing receptors.

	Glutamate			Glycine
	EC₅₀	Hill Slope	n	EC₅₀	Hill Slope	n
	µM			µM
1a/2A	3.42 ± 0.06^#	1.53	6	0.86 ± 0.03^#	1.68	6
1a_C2/1a_C1/2A	3.41 ± 0.13^#	1.46	8
1a_C2/1b_C1/2A	4.16 ± 0.12^*^,#	1.47	6	1.07 ± 0.02^*^,#	1.47	6
1b_C2/1b_C1/2A	5.40 ± 0.14^*	1.37	6
1b/2A	5.11 ± 0.19^*	1.48	6	1.33 ± 0.01^*	1.51	5
1a/2B	1.41 ± 0.12^#	1.63	5	0.29 ± 0.01	1.64	6
1a_C2/1a_C1/2B	1.32 ± 0.06^#	1.52	6
1a_C2/1b_C1/2B	2.09 ± 0.04^*^,#	1.54	6	0.33 ± 0.01^#	1.58	5
1b_C2/1b_C1/2B	2.62 ± 0.04^*	1.49	6
1b/2B	2.93 ± 0.17^*	1.52	6	0.29 ± 0.01	1.57	6

↵* indicates significantly different from 1a/2 and # indicates significantly different from 1b/2 (P < 0.05; one-way ANOVA with Tukey’s post hoc test).