TABLE 2

PK parameters of MTX and 7OH MTX

PK parameters are presented as the mean ± S.D. (n = 5). The raw data were log-transformed and the significant difference analysis was done on the transformed data.

PK ParameterMTX7OH MTX
ControlLEFControlLEF
t1/2 (h)0.53 ± 0.110.41 ± 0.020.91 ± 0.291.26 ± 0.68
Tmax (h)NANA0.80 ± 0.670.75 ± 0.27
Cmax (μg/ml)NANA0.271 ± 0.1431.075 ± 0.320**
AUC (μg/ml*h)9.56 ± 3.3925.8 ± 6.5*0.344 ± 0.0991.558 ± 0.247**
CL (l/h per kilogram)5.64 ± 1.542.05 ± 0.51*NANA
MRT0–t (h)0.42 ± 0.050.49 ± 0.071.26 ± 0.261.22 ± 0.16
  • See the illustration in Fig. 1 for details. CL, clearance; MRT0–t, mean residence time from time zero extrapolated until the end of the dosing interval; NA, not applicable; t1/2, elimination half-life; Tmax, time to reach Cmax.

  • * P < 0.01; **P < 0.001 (differences between two groups were analyzed using the t test).