Primer sequences for Q-PCR analysis
| Gene | Forward | Reverse |
|---|---|---|
| GAPDH | aactttggcattgtggaagg | acacattgggggtaggaaca |
| AOX1 | actgcgtgggccatcttgtctg | ttcctggccgcctatgtgtat |
| Mrp1 | tgcgcttcccactcaacatcc | cgggccaggctcacacg |
| Mrp2 | gtgtggattcccttgggcttt | cacaacgaacacctgcttgg |
| Mrp3 | gcagagacaggcaatgtgaa | gaaagctgacagcatgacca |
| Mrp4 | catcgcggtaaccgtcctc | ccgcagttttactccgcag |
| Mrp5 | gtctcctttcctctcccacatcac | cttgatgcagcctccagatagc |
| Mrp6 | agtcgggaacctgctgaacc | cacgcgctccacggctaccats |
| Bcrp | aaatggagcacctcaacctg | cccatcacaacgtcatcttg |
| P-gp | cagcagtcagtgtgcttacaa | atggctcttttatcggcctca |
| CYP4a10 | tgagggagagctggaaaaga | ctgttggtgatcagggtgtg |
| CYP4a14 | tggggagatcagatccaaag | gacagagtccgccatgattt |
| ACOX1 | caggaagagcaaggaagtgg | cctttctggctgatcccata |
| CPT1 | ccaggctacagtgggacatt | gaacttgcccatgtccttgt |
| Nrf2 | ctttagtcagcgacagaaggac | aggcatcttgtttgggaatgtg |
| Fxr | gcttgatgtgctacaaaagctg | cgtggtgatggttgaatgtcc |
| Pxr | gatggaggtcttcaaatctgcc | cagccggacattgcgtttc |
| Car | ttcaagcctccggcctatct | tgatctgttgcaccataaacgtg |
ACOX1, peroxisomal acyl-coenzyme A oxidase 1; Bcrp, breast cancer resistance protein; CPT1, carnitine palmitoyltransferase I; P-gp, P-glycoprotein; Pxr, pregnane X receptor.