Summary of potential TR response elements in the SLCO2B1 1b and SLCO2B1 1e promoter region
Analysis was performed using the publically available NUBIscan software. Response element marked with † exhibited the highest score in the in silico analysis for this particular motif. Response element highlighted in bold had the highest score in the analysis of the respective promoter fragment. Response element marked with * was previously reported to be the most likely HNF4α binding site, resulting in constitutive activity of the promoter in cells, where this transcription factor is present (Knauer et al., 2013).
| Motif | Sequence | Position |
|---|---|---|
| SLCO2B1-1b promoter | ||
| DR4-III† | GGGTCTgatcAGGTCA | −1756 to −1741 |
| DR4-II | AGGACAgagaAGCCAA | −1078 to −1063 |
| ER6-II | TATCCTgggtggAGGGAA | −1362 to −1345 |
| ER6-I† | AGTCCTcagtccAGGAAA | −899 to −882 |
| DR4-I | AGGCTGgaaaAGGACA | −405 to −390 |
| SLCO2B1-1e promoter | ||
| DR4-IV† | AGCACAtctgAGGCAA | −2170 to −2186 |
| DR1-V | AGAGCAaGGGCCA | −2136 to −2149 |
| ER6-I | TGACCTatttcaGGGTGA | −1241 to −1223 |
| DR4-III | AGGTGGgagaAGGTCA | −1222 to −1206 |
| DR1-IV | AGGTCTgAGGCCT | −996 to 983 |
| DR1-III | AGGACAgATCTCA | −954 to −941 |
| DR1-II | AGCCCAcAGGAAA | −167 to −155 |
| DR4-II | AGGGAAgcacTGGCC | −55 to −40 |
| DR4-1 | TGGGCAggggAGGGAA | −46 to −30 |
| DR1-I†* | AGGGCAaAGTCCA | −17 to −4 |