Temperature dependence of drug-block rates
The calculated time course of drug washout (τoff), rate of drug binding (kon), and rate of drug dissociation (koff) at 22°C and 36°C was derived using a simple bimolecular model of hERG drug binding.
| τoff (s) | 22°C | 36°C | ||||
|---|---|---|---|---|---|---|
| 22°C | 36°C | kon | koff | kon | koff | |
| s | s | M−1•s−1 | s−1 | M−1•s−1 | s−1 | |
| Verapamil | 8.89 | 1.60 | 7.51 × 105 | 0.113 | 1.78 × 106 | 0.626 |
| Cisapride | 17.15 | 6.46 | 1.10 × 106 | 0.058 | 7.71 × 106 | 0.155 |
| Bepridil | 5.52 | 3.86 | 1.32 × 106 | 0.181 | 3.74 × 106 | 0.259 |
| Terfenadine | 217.39 | 116.28 | 8.80 × 105 | 0.005 | 4.73 × 106 | 0.009 |