WT | N410K+T423K | |||||
---|---|---|---|---|---|---|

pK_{B}^{a} | Logαβ_{ACh}^{b} | n^{c} | pK_{B} | Logαβ_{ACh} | n | |

PLA | 7.00 ± 0.05 | −3^{#} | 3 | 6.41 ± 0.25^{*} | −0.70 ± 0.56^{*} | 3 |

MBP | 6.54 ± 0.29 | −3^{#} | 4 | n.a. | n.a. | 4 |

Protamine | 6.01 ± 0.25 | −0.86 ± 0.19 | 4 | n.a. | n.a. | 4 |

n.a., not applicable, as no alteration of the EC

_{50}of ACh was observed, and therefore, no estimate could be provided.↵

Negative logarithm of the allosteric modulator equilibrium dissociation constant.^{a}↵

Logarithm of the cooperativity factor for the interaction between the peptides and ACh.^{b}↵

Number of experiments performed.^{c}↵* Significantly different from the corresponding value at the wild type (

*P*< 0.05), Student’s*t*test.↵

Interaction was indistinguishable from competition (F-test), indicative of very high negative cooperativity (^{#}*αβ*→ 0), and thus the cooperativity factor was constrained to an arbitrarily low value.