Mutant | EC50 ± S.E. (µM) (Hill Coefficient ± S.E.) | n | Fold Change |
---|---|---|---|
C224A | 32.56 ± 2.29 (1.37 ± 0.11) | 7 | 1 |
H142C | 2.70 ± 0.22 (1.44 ± 0.07)a | 7 | 0.1 |
N143C | 199.69 ± 22.86 (1.33 ± 0.11)a | 7 | 6.1 |
S144C | 133.76 ± 11.96 (1.31 ± 0.07)a | 7 | 4.1 |
F145C | 383.53 ± 114.30 (1.40 ± 0.04) | 7 | 11.8 |
L146C | 361.39 ± 42.59 (1.30 ± 0.04)a | 7 | 11.1 |
R147C | 90.32 ± 6.54 (1.82 ± 0.16)a | 8 | 2.8 |
I148C | 19.37 ± 2.62 (1.53 ± 0.13) | 8 | 0.6 |
D149C | 39.79 ± 7.11 (2.15 ± 0.27) | 7 | 1.2 |
S150C | 54.39 ± 6.95 (1.78 ± 0.13) | 7 | 1.7 |
D151C | 36.11 ± 3.25 (1.43 ± 0.08) | 7 | 1.1 |
G152C | 54.91 ± 5.01 (1.48 ± 0.06) | 7 | 1.7 |
T153C | 122.21 ± 10.01 (1.71 ± 0.08)a | 6 | 3.8 |
V154C | 104.05 ± 5.10 (1.51 ± 0.14)a | 7 | 3.2 |
Y155C | 111.49 ± 10.68 (1.64 ± 0.09)a | 7 | 3.4 |
T156C | 628.48 ± 50.61 (1.71 ± 0.06)a | 6 | 19.3 |
S157C | 3683.50 ± 413.66 (1.43 ± 0.09)a | 8 | 113.1 |
Q158C | 35.44 ± 6.63 (1.44 ± 0.10) | 7 | 1.1 |
R159C | 3102.33 ± 130.43 (1.53 ± 0.03)a | 6 | 95.3 |
↵a Values statistically significantly different from C224A (P < 0.001).