WT | N410K+T423K | na | |
---|---|---|---|
Equilibrium pKDb | 10.18 ± 0.10 | 10.27 ± 0.21 | 5 |
koff (min−1)c | 0.037 ± 0.002 | 0.007 ± 0.001* | 3 |
t1/2 (min)d | 18.7 | 105 | 3 |
kon (M−1.min−1)e | 2.91 ± 0.26 × 108 | 0.90 ± 0.17 × 108* | 3 |
Kinetic pKDf | 9.93 ± 0.28 | 10.36 ± 0.22 | n.a. |
n.a., not applicable, as kinetic pKD values were calculated from kinetic rate constants kon and koff.
↵a Number of experiments performed for each assay.
↵b Negative logarithm of [3H]NMS equilibrium dissociation constant derived from saturation binding assays.
↵c Dissociation rate constant of [3H]NMS derived from dissociation binding assays.
↵d Half-life dissociation estimate of [3H]NMS calculated from koff estimates.
↵e Association rate constant of [3H]NMS derived from association binding assays.
↵f Negative logarithm of [3H]NMS equilibrium dissociation constant calculated from koff/kon.
↵* Significantly different from the corresponding value at the wild type (P < 0.05), Student’s t test.