WT | N410K+T423K | n^{a} | |
---|---|---|---|

Equilibrium pK_{D}^{b} | 10.18 ± 0.10 | 10.27 ± 0.21 | 5 |

k (min_{off}^{−1})^{c} | 0.037 ± 0.002 | 0.007 ± 0.001^{*} | 3 |

t_{1/2} (min)^{d} | 18.7 | 105 | 3 |

k (M_{on}^{−1}.min^{−1})^{e} | 2.91 ± 0.26 × 10^{8} | 0.90 ± 0.17 × 10^{8}^{*} | 3 |

Kinetic pK_{D}^{f} | 9.93 ± 0.28 | 10.36 ± 0.22 | n.a. |

n.a., not applicable, as kinetic

*pK*values were calculated from kinetic rate constants_{D}*k*and_{on}*k*._{off}↵

Number of experiments performed for each assay.^{a}↵

Negative logarithm of [^{b}^{3}H]NMS equilibrium dissociation constant derived from saturation binding assays.↵

Dissociation rate constant of [^{c}^{3}H]NMS derived from dissociation binding assays.↵

Half-life dissociation estimate of [^{d}^{3}H]NMS calculated from*k*estimates._{off}↵

Association rate constant of [^{e}^{3}H]NMS derived from association binding assays.↵

Negative logarithm of [^{f}^{3}H]NMS equilibrium dissociation constant calculated from*k*/_{off}*k*._{on}↵* Significantly different from the corresponding value at the wild type (

*P*< 0.05), Student’s*t*test.