ACKR3 mRNA and protein detection in mammalian tissue
| Mouse/Rodent | Human | ||||||
|---|---|---|---|---|---|---|---|
| System | Cell Type | Origin | mRNA | Protein | mRNA | Protein | References |
| Haemato-poietic system | B cells | Peripheral blood, BM, and/or lymphoid organs | RNAseq | FC | Transcriptomics, RT-PCR | FC, IF | Infantino et al. (2006), Sierro et al. (2007), Heng et al. (2008), Tarnowski et al. (2010), Biajoux et al. (2012), Wang et al. (2012), Melo et al. (2014), Alampour-Rajabi et al. (2015) |
| T cells (CD4+, regulatory, helper, memory) | RNAseq | Transcriptomics, RT-PCR | FC, IF | Balabanian et al. (2005), Infantino et al. (2006), Sierro et al. (2007), Heng et al. (2008), Tarnowski et al. (2010), Biajoux et al. (2012), Melo et al. (2014) | |||
| Innate lymphoid cells | RNAseq | Heng et al. (2008) | |||||
| NK cells | Transcriptomics, RT-PCR | Infantino et al. (2006), Sierro et al. (2007) | |||||
| Dendritic cells | RNAseq | Transcriptomics, RT-PCR | FC, IF | Infantino et al. (2006), Sierro et al. (2007), Heng et al. (2008) | |||
| Monocytes | RT-PCR | FC, IF | Infantino et al. (2006), Tarnowski et al. (2010), Chatterjee et al. (2015) | ||||
| Neutrophils | RNAseq | Heng et al. (2008) | |||||
| Macrophages | RNAseq | FC | Heng et al. (2008), Chatterjee et al. (2015) | ||||
| Basophils | RT-PCR | FC | Infantino et al. (2006) | ||||
| Mesenchymal stem cells | BM | RT-qPCR | Transcriptomics, RT-PCR | Kim et al. (2016), Liu et al. (2018), Kuçi et al. (2019) | |||
| Circulatory / lymphatic system | ND | Whole (heart) | Lac-Z reporter, northern blot | Northern blot | Burns et al. (2006), Gerrits et al. (2008), Berahovich et al. (2014) | ||
| Venous endothelium | Digestive tract, heart, kidney, lung, liver, lymphoid organs | Lac-Z reporter (except liver) | IHC | Berahovich et al. (2014) | |||
| Vascular smooth muscle cells | Heart, nervous system, kidney, digestive tract, skeletal muscle, lymphoid organs | RT-PCR | IHC | Neusser et al. (2010), Rajagopal et al. (2010), Berahovich et al. (2014) | |||
| Sinusoidal cells | Spleen | IHC | IHC | Berahovich et al. (2014) | |||
| Endothelial cells | Kidney | IHC | Neusser et al. (2010) | ||||
| ND | Kidney lymphatic vessels | IF | Neusser et al. (2010) | ||||
| ND | Vena cava | RT-qPCR | Klein et al. (2014) | ||||
| ND | Thoracic duct | RT-qPCR | Klein et al. (2014) | ||||
| Nervous system | ND | Brain (whole) | Lac-Z reporter, ISH, northern blot | Northern blot | Burns et al. (2006), Gerrits et al. (2008), Thelen and Thelen (2008), Yu et al. (2011), Berahovich et al. (2014) | ||
| ND | Brain vasculature | EGFP reporter, ISH | Schönemeier et al. (2008), Cruz-Orengo et al. (2011) | ||||
| Astrocytes | Brain | IHC | FC (intracellular) | Calatozzolo et al. (2011), Puchert et al. (2017) | |||
| Respiratory system | ND | Lung (whole) | RT-PCR, northern blot | IF | Northern blot | Burns et al. (2006), Berahovich et al. (2014), Cao et al. (2016), Konrad et al. (2017), Ngamsri et al. (2017) | |
| ND | Lung vessels | Lac-Z reporter | Gerrits et al. (2008) | ||||
| Secretory system | ND | Kidney (whole) | Northern blot | RT-qPCR, northern blot | IHC | Burns et al. (2006), Neusser et al. (2010), Maishi et al. (2012), Berahovich et al. (2014) | |
| Renal multipotent progrenitors | Patient-derived healthy kidney tissue | RT-qPCR | IF | Mazzinghi et al. (2008) | |||
| ND | Kidney tubules | Lac-Z reporter | IHC | IHC | Neusser et al. (2010), Berahovich et al. (2014) | ||
| ND | Kidney glomeruli | Lac-Z reporter | Gerrits et al. (2008) | ||||
BM, bone marrow; FC, flow cytometry; IF, immunofluorescence; ISH, in situ hybridization; IHC, immunohistochemistry; ND, not determined; NK, natural killer; RNAseq, RNA sequencing; RT-PCR, reverse transcription polymerase chain reaction; RT-qPCR, real time quantitative polymerase chain reaction.