Regular ArticleOrientation of Caffeine within the Active Site of Human Cytochrome P450 1A2 Based on NMR Longitudinal (T1) Relaxation Measurements
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2008, Archives of Biochemistry and BiophysicsCitation Excerpt :Likewise, since the degree of dapsone activation of flurbiprofen metabolism differed among the CYP2C9 variants as compared to wild-type protein, then perhaps these differences in activation could be explained by differences in the change of proton–heme distances due to the presence of dapsone would be observed among the proteins. T1 relaxation studies have been used successfully to estimate distances for codeine in CYP2D6 [20], diclofenac in CYP2C9 [19], caffeine in CYP1A2 [16], flurbiprofen and dapsone in CYP2C9 [12] and acetaminophen and caffeine in CYP3A4 [18]. The present study demonstrated that in all CYP2C9 proteins studied (CYP2C9.1, CYP2C9.2, CYP2C9.3, and CYP2C9.5) the flurbiprofen proton to heme-iron distances, notably at the 4′-H which is the primary site of metabolism, though variable, did not directly correlate with previously observed differences in enzyme activity and correspondingly not directly with the amino acid substitutions in the variant proteins, either.
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Current address: Department of Pharmacology, University of Michigan, 1150 W. Medical Center Drive, Ann Arbor, MI 48109.
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