Regular Article
Oxidative Stress and Heat Shock Stimulate RGS2 Expression in 1321N1 Astrocytoma Cells

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Abstract

RGS2, a regulators of G-protein signaling family member, regulates G-protein signaling and is itself controlled in part by regulated expression. We tested if cell stress regulates RGS2 expression in human astrocytoma 1321N1 cells. Treatment with H2O2 increased RGS2 mRNA levels time- and concentration-dependently, with 200 μM H2O2 causing an approximately eightfold increase after 2 h. Peroxynitrite and heat shock also increased RGS2 mRNA levels. H2O2-induced RGS2 expression was negatively regulated by phosphoinositide-3-kinase and extracellular signal-regulated kinases. H2O2 also concentration-dependently increased RGS2 protein levels, and the RGS2 appeared to be predominantly in the nucleus. These results demonstrate that RGS2 expression is up-regulated by cell stress.

References (30)

  • H.G. Dohlman et al.

    J. Biol. Chem.

    (1997)
  • Berman, D. M., and Gilman, A. G.J. Biol. Chem.273,...
  • L. De Vries et al.

    Annu. Rev. Pharmacol. Toxicol.

    (2000)
  • D.P. Siderovski et al.

    DNA Cell Biol.

    (1990)
  • D.P. Siderovski et al.

    DNA Cell Biol.

    (1994)
  • T. Ingi et al.

    J. Neurosci.

    (1998)
  • S.A. Burchett et al.

    J. Neurochem.

    (1998)
  • S.A. Burchett et al.

    J. Neurochem.

    (1999)
  • D.J. Pepperl et al.

    Biochem. Biophys. Res. Commun.

    (1998)
  • C. Beadling et al.

    J. Immunol.

    (1999)
  • L. Song et al.

    J. Biol. Chem.

    (1999)
  • S.L. Grant et al.

    Mol. Pharmacol.

    (2000)
  • S.P. Heximer et al.

    DNA Cell Biol.

    (1997)
  • C.C. Tseng et al.

    Endocrinology

    (1998)

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