Elsevier

Analytical Biochemistry

Volume 264, Issue 2, 15 November 1998, Pages 180-184
Analytical Biochemistry

Regular Article
Measurement of PlasmaS-Adenosylmethionine andS-Adenosylhomocysteine as Their Fluorescent Isoindoles

https://doi.org/10.1006/abio.1998.2839Get rights and content

Abstract

The low levels ofS-adenosylmethionine (AdoMet) andS-adenosylhomocysteine (AdoHcy) in plasma can be measured by formation of the fluorescent isoindole derivatives of these compounds. The procedure involves an initial separation of AdoMet and AdoHcy in deproteinized plasma by HPLC on a C-8 column followed by derivatization with naphthalenedialdehyde and cyanide for 10 min at pH 9.0. The fluorescent derivatives of AdoMet and AdoHcy are then chromatographed by HPLC on a C-18 column monitored with a fluorescence monitor. The formation of the isoindole goes to 85% completion and the overall recovery of standards added to plasma is about 65%. Correction for recovery is made by addition of known quantities of AdoMet and AdoHcy to plasma. Normal values (± SE) for AdoMet were 102.7 nM ± 9.9 and for AdoHcy were 22.7 ± 3.1.

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    Supported by NIH Grants DK15289 and DK 46788 and by the Medical Research Service of the Department of Veterans Affairs.

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