Regular Article
Evidence That Human Bone Cells in Culture Contain Binding Sites for Osteogenic Protein-1

https://doi.org/10.1006/bbrc.1994.1824Get rights and content

Abstract

Recent studies have shown that osteogenic protein (OP)-1 or bone morphogenetic protein (BMP)-7 increases proliferation and differentiation of human bone cells (HBCs) in culture and modulates production of IGF system components. In order to study the mechanism by which OP-1 causes these effects, we sought to test the hypothesis that the effects of OP-1 are mediated at least in part by specific receptors (for OP-1) in HBCs. Binding studies with serum-free cultures of normal HBCs and human osteosarcoma cells showed a maximum binding of 15 - 25% for [125I]OP-1; the binding was time- and temperature-dependent in different experiments. Scatchard analysis of [125]OP-1 binding to TE85 human osteosarcoma cells showed at least two binding sites, about 30,000 and 60,000 per cell with apparent Kd of 2.5 × 10−10M and 1 × 10−9M, respectively. [125]OP-1 binding to TE85 cells was displaced by unlabeled OP-1 (16-1000 ng/ml) with a 50% displacement at 250 ng/ml. BMP-2 effectively displaced [125]OP-1 binding to HBCs while TGF-β1 did not. Affinity cross-linking studies showed that [125]OP-1 interacted specifically with three binding sites with apparent Mr of 34, 65 and > 205kDa. The findings of this study demonstrate that the effects of OP-1 on HBCs may be mediated in part via BMP-specific receptors.

References (0)

Cited by (13)

  • Growth factors, cytokines, and pediatric malignant primary bones tumors

    2021, Bone Cancer: Bone Sarcomas and Bone Metastases - From Bench to Bedside
  • In vivo BMP-7 (OP-1) enhancement of osteoporotic vertebral bodies in an ovine model

    2006, Spine Journal
    Citation Excerpt :

    OP-1 in the adult skeleton acts as a potent differentiation factor inducing an osteogenic process [20,21]. The OP-1 interacts with specific receptors on the surface of mesenchymal progenitor cells, recruiting them to the site of application and stimulating the cells to differentiate to mature osteoblasts [22,23]. Theoretical advantages of local treatment of osteoporotic vertebra with BMPs include the potential for inducing a rapid increase in bone strength specifically in vertebra thought to be at particular risk for fracture.

View all citing articles on Scopus
View full text