Regular Article
Characterization and Chromosomal Localization of the Human A2a Adenosine Receptor Gene: ADORA2A

https://doi.org/10.1006/bbrc.1996.0916Get rights and content

Abstract

The gene for the stimulatory G protein-coupled human A2a adenosine receptor was isolated and sequence analysis revealed two exons that are interrupted by an intron of approximately 6.4 kb. An intron is located in the same region in the human A1 and A2b adenosine receptor genes. Comparison of the A2a genomic and cDNA sequences reveals two nucleotide differences in the coding region and the presence of an aberrant sequence in the 5′208 base pairs of the A2a cDNA including a polymorphism in the third base of codon Tyr-361 and a Gly codon which was always detected at residue 392, indicating that the Arg codon present in the cDNA may be an artifact. Fluorescentin situhybridization and PCR analysis of human-hamster hybrid cell panels shows that the A2a receptor gene is localized to chromosome 22q11.2. This is in contrast with previous reports (subsequently retracted) which mapped the A2a gene to chromosome 11q11–13.

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    Citation Excerpt :

    Accordingly, the corresponding gene (ADORA2A, NM_000675) is discussed as a promising candidate for increased anxiety. ADORA2A is located on chromosome 22q11.23 and has two coding exons spanning about 9 kb (MacCollin et al., 1994; Peterfreund et al., 1996; Le et al., 1996). Mutation screening of these exons revealed several variants (Deckert et al., 1998, 2001) of which SNP rs5751876 (1976C/T) was associated or linked with PD (Deckert et al., 1998; Hamilton et al., 2004).

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Corresponding author: Fax +61 2 295 9281; email [email protected].

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