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Molecular Cloning of the Rat Inducible Nitric Oxide Synthase Gene Promoter,☆☆

https://doi.org/10.1006/bbrc.1996.0968Get rights and content

Abstract

The inducible nitric oxide synthase (iNOS) is not constitutively expressed but is induced in many types of mammalian cells by cytokines and bacterial endotoxins. Previously, we have reported the most interesting feature of rat iNOS gene that is up-regulated by cyclic AMP at transcriptional level as demonstrated by nuclear run-on assay. We now have isolated and sequenced the rat iNOS gene promoter and determined the transcription start site. Moreover, we have constructed a rat iNOS promoter-chloramphenicol acetyltransferase (CAT) fusion gene and analyzed its inducibility by interleukin 1β and cyclic AMP in transiently transfected Swiss 3T3 fibroblasts.

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The nucleotide sequence data reported in this paper have been submitted to the EMBL/Gen Bank database under the Accession Number X95629.

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Abbreviations:iNOS, inducible nitric oxide synthase; NO, nitric oxide; CAT, chloramphenicol acetyltransferase; IL-1β,interleukin 1β.

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