Biochemical and Biophysical Research Communications
Regular ArticleInsulin Suppresses the Induction of CYP2B1 and CYP2B2 Gene Expression by Phenobarbital in Adult Rat Cultured Hepatocytes☆
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Cited by (35)
Nuclear receptor phosphorylation in xenobiotic signal transduction
2020, Journal of Biological ChemistryCitation Excerpt :In addition to EGFR, PB can also bind the insulin receptor to repress ERK1/2 activation (71). Insulin has long been known to repress PB induction of CYP2B, and, conversely, diabetic livers increased CYP expressions (72–75). This PB-insulin interaction can now be understood by its competitive antagonism via the insulin receptor.
Phenobarbital reduces blood glucose and gluconeogenesis through down-regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression in rats
2015, Biochemical and Biophysical Research CommunicationsLipid components prepared from a freshwater Clam (Corbicula fluminea) extract ameliorate hypercholesterolaemia in rats fed high-cholesterol diet
2013, Food ChemistryCitation Excerpt :Expression was calculated using the standard curve method. Because the apo E mRNA level was not affected by any treatment used in the present study (data not shown), we used it as the normalisation standard (Oda, Nozawa, Hitomi, & Kakinuma,1995; Yoshida, Kimura, Oda, & Kakinuma, 1996). Previous studies demonstrated that the amounts of the CYP7A1 and ABCG5 mRNA have a good correlation with the activities or the protein levels (Noshiro, Mishimoto, & Okuda, 1990; Yu et al., 2005).
Cell shape, cell-cell contact, cell-extracellular matrix contact and cell polarity are all required for the maximum induction of CYP2B1 and CYP2B2 gene expression by phenobarbital in adult rat cultured hepatocytes
2008, Biochemical PharmacologyCitation Excerpt :At present, no established cell lines that respond well to PB treatment exist, although it has been reported that endogenous CYP2B1/2B2 can be induced in primary rat hepatocytes [6]. Various efforts have been made so far to observe sufficient induction of CYP2B1/2B2 in cultured hepatocytes [3,6–9], and the most successful methods have revolved around the manipulation of extracellular matrices. Several studies have established a method whereby primary hepatocytes can be reproducibly cultured on EHS gel (a reconstituted extracellular basement membrane gel from mouse Engelbreth-Holm-Swarm sarcoma) [6–12].
AMP-activated protein kinase mediates phenobarbital induction of CYP2B gene expression in hepatocytes and a newly derived human hepatoma cell line
2005, Journal of Biological ChemistryApolipoprotein A-I gene expression is upregulated by polychlorinated biphenyls in rat liver
2000, Journal of Nutritional Biochemistry
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Abbreviations: apo, apolipoprotein; ANOVA, analysis of variance; CYP, cytochrome P-450; Dex, dexamethasone; EHS, Engelbreth-Holm-Swarm; IRS, insulin responsive sequence; IRS1, insulin receptor substrate 1; PB, phenobarbital; PEPCK, phosphoenolpyruvate carboxykinase; PI-3 kinase, phosphatidylinositol-3 kinase; TAT, tyrosine aminotransferase; TIC, type I collagen; UGT, UDP-glucuronosyltransferase.
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