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Evidence That Acetyl-CoA Carboxylase Isoforms Play Different Biological Roles in H9c2 Cardiaomyocyte

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Abstract

The present work was performed to identify the possible roles of acetyl-CoA carboxylase isoforms (ACC-α and ACC-β). Two forms show 70% amino acid identity, but N-terminal regions share no homology, indicating that these may be uniquely related to the specific role of each ACC form. Thus, we investigated whether introduction of the exogenous ACC N-terminus into H9c2 cardiomyocytes that express both ACC forms causes a noticeable change in a specific pathway of fatty acid metabolism. The effect of ACC-α N-terminus overexpression was specific to the fatty acid synthesis rate resulting in an 80% induction, whereas overexpression of the ACC-β N-terminus increased fatty acid oxidation rate 50% without affecting the fatty acid synthesis rate. These results suggest that ACC-α and β are involved in the regulation of fatty acid synthesis and oxidation, respectively, and that the N-terminus plays an important role in the process. We further demonstrated that novel proteins specifically bound to the ACC N-terminus. This interaction may mediate the involvement of each ACC form in different cellular activities.

References (40)

  • K.G. Thampy

    J. Biol. Chem.

    (1989)
  • A. Bianchi et al.

    J. Biol. Chem.

    (1990)
  • M.D. Lane et al.

    Curr. Top. Cell. Regul.

    (1974)
  • G.A. Cook

    J. Biol. Chem.

    (1984)
  • J.D. McGarry et al.

    J. Biol. Chem.

    (1978)
  • L. Abu-Elheiga et al.

    J. Biol. Chem.

    (1997)
  • J.D. McGarry et al.

    J. Biol. Chem.

    (1978)
  • N. Kudo et al.

    J. Biol. Chem.

    (1995)
  • K. Park et al.

    J. Biol. Chem.

    (1991)
  • M.J. Arslanian et al.

    Methods Enzymol

    (1975)
  • A.W. Alberts et al.

    J. Biol. Chem.

    (1974)
  • J. Ha et al.

    J. Biol. Chem.

    (1994)
  • N.B. Beaty et al.

    J. Biol. Chem.

    (1983)
  • J.B. Allred et al.

    Biochem. Biophys. Res. Comm.

    (1985)
  • M.J.H. Geelen et al.

    Biochem. Biophys. Res. Comm.

    (1997)
  • P.A. Srere et al.

    FEBS Lett.

    (1990)
  • G.R. Welch et al.

    J. Theor. Biolo.

    (1991)
  • R.G. Shulman

    Trends Biochem. Sci.

    (1991)
  • S. Numa et al.

    Fatty Acid Metabolism and Its Regulation

    (1984)
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    Numa, S.

    1

    To whom correspondence should be addressed. Department of Molecular Biology, College of Medicine, Kyung Hee University, Seoul 130-701, Korea, Fax: 82-2-959-8168. E-mail:[email protected].

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