Regular ArticleRecombinant Expression of a Selective Blocker of M1 Muscarinic Receptors
References (16)
- et al.
A snake toxin against muscarinic acetylcholine receptors: Amino acid sequence, subtype specificity, and effect on guinea-pig ileum
Toxicon
(1995) - et al.
Purification and sequence determination of a new muscarinic toxin (MT4) from the venom of the green mamba (Dendroaspis angusticeps)
Toxicon
(1995) - et al.
A toxin from the green mamba Dendroaspis angusticeps: Amino acid sequence and selectivity for muscarinic m4 receptors
FEBS Lett.
(1994) - et al.
Amino acid sequence of a muscarinic toxin deduced from the cDNA sequence
Toxicon
(1991) - et al.
The second intracellular loop of the alpha2-adrenergic receptors determines subtype-specific coupling to cAMP production
J. Biol. Chem.
(1997) - et al.
Muscarinic toxins from Dendroaspis (mamba) venom: Peptides selective for subtypes of muscarinic acetylcholine receptors. Comprehensive summaries of Uppsala dissertations from the Faculty of Science and Technology
(1996)
Cited by (31)
Different roles for M<inf>1</inf> and M<inf>2</inf> receptors within perirhinal cortex in object recognition and discrimination
2014, Neurobiology of Learning and MemoryCitation Excerpt :M1 receptor function in PRh was also assessed using a muscarinic snake toxin, MT-7 (also called MTX7 and m1-toxin1). Although pirenzepine is considered an M1 specific antagonist, and its use allows comparisons with previous studies using this compound, the selectivity of pirenzepine for M1 opposed to M4 receptors is only 4–6 times greater (Madison, Jones, Tom-Moy, & Brown, 1987; Nasman, Jolkkonen, Ammoun, Karlsson, & Akerman, 2000). MT-7 is one of the most selective ligands available for targeting M1 receptor subtypes (Olianas, Adem, Karlsson, & Onali, 2004); it has more than 20,000 times higher selectivity for M1 receptors than any other muscarinic receptor subtype (Karlsson, Jolkkonen, Mulugeta, Onali, & Adem, 2000).
Glycosylphosphatidylinositol (GPI)-anchoring of mamba toxins enables cell-restricted receptor silencing
2012, Biochemical and Biophysical Research CommunicationsCitation Excerpt :The toxin constructs in pFastBac1 were used to generate recombinant baculovirus using the Bac-to-Bac expression system (Invitrogen). Baculoviruses for untagged receptor expression have been described earlier [6,9,20]. The enhanced Green Fluorescent Protein (eGFP)-tagged M1 receptor was generated by fusion of the M1 receptor cDNA to eGFP in pEGFP-N1 (BD Biosciences Clontech, Palo Alto, CA) after removal of the M1 stop codon.
Biochemistry of Envenomation
2012, Advances in Clinical ChemistryMuscarinic toxins
2011, ToxiconCitation Excerpt :At the toxin level, various expression systems have been tested for toxin recombinant expression. A baculovirus vector-Sf9 insect cell expression system (Nasman et al., 2000) and a yeast expression in P. pastoris (Krajewski et al., 2001) have been used to obtain recombinant MT7 but at relatively low levels: 0.9 mg/l and 0.15 mg/l, respectively. In the yeast expression system, two mutants were generated and Phe38 was found to be involved in the stability of the MT7-M1 receptor interaction (Krajewski et al., 2001).
Differential involvement of M1-type and M4-type muscarinic cholinergic receptors in the dorsomedial striatum in task switching
2008, Neurobiology of Learning and Memory
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