Original ArticleCloning and Expression of Fibroblast Growth Factor Receptor-1 Isoforms in the Mouse Heart: Evidence for Isoform Switching During Heart Development
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Real-ambient particulate matter exposure-induced FGFR1 methylation contributes to cardiac dysfunction via lipid metabolism disruption
2023, Science of the Total EnvironmentThe FGF21 Receptor Signaling Complex: Klothoβ, FGFR1c, and Other Regulatory Interactions
2016, Vitamins and HormonesCitation Excerpt :Conversely, malignant astrocytomas have been shown to express both FGFR1α and FGFR1β isoforms, while normal fetal and adult brain express only FGFR1α (Yamaguchi, Saya, Bruner, & Morrison, 1994). Similarly, while the “long” (α) isoform of FGFR1 was expressed in embryonic mouse heart, only the “short” (β) FGFR1 isoform was detected in adult heart (Jin et al., 1994). Similarly, FGFR2α and FGFR2β isoforms have also been shown to be differentially expressed during amphibian development (Shi, Launay, Fromentoux, Feige, & Boucaut, 1994).
Leupeptin enhances cell surface localization of fibroblast growth factor receptor 1 in adult sensory neurons by increased recycling
2012, European Journal of Cell BiologyCitation Excerpt :Collectively, our data indicate that enhanced recycling of FGFR1 strongly promotes axonal elongation, but not axonal branching, of adult DRG neurons in vitro. We used FGFR1 IIIc (three Ig-like domains in the extracellular domain) coupled to EGFP (FGFR1-EGFP; Jin et al., 1994) for transfection of PC12 pheochromocytoma cells and dissociated DRG neuron cultures. Stable PC12 cell FGFR1-EGFP transfectants were generated as described before (Hausott et al., 2008; clone L1 was used for this study) and cultured in collagen-coated optical plastic dishes (Ibidi, Austria) under standard conditions in a humidified atmosphere at 37 °C with 5% CO2.
Inhibition of fibroblast growth factor receptor 1 endocytosis promotes axonal branching of adult sensory neurons
2011, NeuroscienceCitation Excerpt :FGFR1 IIIc (three Ig-like domains in the extracellular domain) coupled to EGFP (FGFR1-EGFP; Jin et al., 1994) was used for transfection of PC12 cells and DRG neurons.
Endothelium-targeted overexpression of constitutively active FGF receptor induces cardioprotection in mice myocardial infarction
2009, Journal of Molecular and Cellular CardiologyCitation Excerpt :The biological effects of extracellular FGF2 are mediated by binding to high-affinity FGFR1–4 of the tyrosine kinase family [11]. FGFR-1 is the best studied FGFR in embryonic, neonatal, and adult cardiomyocytes [12,13]. Activated FGFRs recruit and phosphorylate other signaling molecules, culminating in the activation of major signal transduction pathways such as all three branches of the mitogen activated protein kinase (MAPK pathway), the phospholipase C/protein kinase C (PKC)-, and PI3-kinase/Akt- and Src-associated pathways [14], leading to cell proliferation and differentiation, whereas FGFR also transmits negative cell proliferative effects by inhibition of the Ras/MAPK pathway or induction of cell cycle inhibitors [15].
Promotion of neurite outgrowth by fibroblast growth factor receptor 1 overexpression and lysosomal inhibition of receptor degradation in pheochromocytoma cells and adult sensory neurons
2008, NeuroscienceCitation Excerpt :RPMI 1640 cell culture medium (Gibco Invitrogen, Lofer, Austria) supplemented with 10% horse serum (Gibco Invitrogen), 5% fetal bovine serum (Gibco Invitrogen) and antibiotic-antimycotic (100 units/ml penicillin, 100 μg/ml streptomycin, 0.25 μg/ml amphotericin, Gibco Invitrogen) was changed two times a week. FGFR1S (FGFR1β, two Ig-like domains, IgIIIc) and FGFR1L (FGFR1α, three Ig-like domains, IgIIIc) fused to EGFP (gifts from Dr. P. Cattini; the study was supported by the Medical University Innsbruck, Christoph-Probst-Platz Innrain 52, A-6020 Innsbruck; Jin et al., 1994) were applied for transfection. PC12 cells were transfected using the Amaxa Nucleofector™ (program U-29).