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The Subcellular Distribution of GABARAP and Its Ability to Interact with NSF Suggest a Role for This Protein in the Intracellular Transport of GABAA Receptors

https://doi.org/10.1006/mcne.2001.1005Get rights and content

Abstract

GABAA receptors the major sites of fast synaptic inhibition in the brain are composed predominately of α, β, and γ2 subunits. The receptor γ2 subunit interacts with a 17-kDa microtubule associated protein GABARAP, but the significance of this interaction remains unknown. Here we demonstrate that GABARAP, which immunoprecipitates with GABAA receptors, is not found at significant levels within inhibitory synapses, but is enriched within the Golgi apparatus and postsynaptic cisternae. We also demonstrate that GABARAP binds directly to N-ethylmaleimide-sensitive factor (NSF), a protein critical for intracellular membrane trafficking events. NSF and GABARAP complexes could be detected in neurons and these two proteins also colocalize within intracellular membrane compartments. Together our observations suggest that GABARAP may play a role in intracellular GABAA receptor transport but not synaptic anchoring, via its ability to interact with NSF. GABARAP may therefore have an important role in the production of GABAergic synapses.

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    To whom correspondence should be addressed at MRC-LMCB, University College, Gower Street, London WC1E 6BT, UK. Fax: 02076797805. E-mail: [email protected].

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