Abstract
The development of prostate cancer has been linked to high level of dietary fat intake. Our laboratory investigates the connection between cancer cell growth and fatty acid products. Studying human prostatic carcinoma PC-3 cells, we found that prostaglandin E2 (PGE2) increased cell growth and up-regulated the gene expression of its own synthesizing enzyme, cyclooxygenase-2 (COX-2). PGE2 increased COX-2 mRNA expression dose-de-pendently with the highest levels of stimulation seen at the 3-hour period following PGE2 addition. The NSAID flurbiprofen (5 uM), in the presence of exogenous PGE2, inhibited the up-regulation of COX-2 mRNA and cell growth. These data suggest that the levels of local intracellular PGE2 play a major role in the growth of prostate cancer cells through an activation of COX-2 gene expression.
Keywords
- Human Prostate Cancer Cell Line
- Experimental Procedure Section
- Malignant Prostatic Tissue
- Human Malignant Prostatic Tissue
- UCSF Cell Culture Facility
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© 1997 Springer Science+Business Media New York
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Tjandrawinata, R.R., Hughes-Fulford, M. (1997). Up-Regulation of Cyclooxygenase-2 by Product-Prostaglandin E2 . In: Honn, K.V., Marnett, L.J., Nigam, S., Jones, R.L., Wong, P.YK. (eds) Eicosanoids and other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury 3. Advances in Experimental Medicine and Biology, vol 407. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1813-0_25
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DOI: https://doi.org/10.1007/978-1-4899-1813-0_25
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