Skip to main content
Log in

Modulation by oxotremorine and atropine of acetylcholine release evoked by electrical stimulation of the myenteric plexus of the guinea-pig ileum

  • Published:
Naunyn-Schmiedeberg's Archives of Pharmacology Aims and scope Submit manuscript

Summary

  1. 1.

    The effects of oxotremorine and atropine on the release of acetylcholine from longitudinal muscle strips of the guinea-pig ileum stimulated at frequencies between 0.1 and 3 Hz in the presence of eserine were investigated. In control experiments the acetylcholine output per stimulus declined with increasing frequencies of stimulation.

  2. 2.

    Oxotremorine inhibited the release of acetylcholine in a concentration-dependent fashion. At a concentration of 10−6 M oxotremorine, the release evoked by 0.1 Hz was reduced by 54%. With increasing frequencies of stimulation the inhibitory effect of oxotremorine became smaller.

  3. 3.

    Atropine enhanced the output of acetylcholine evoked by electrical stimulation. In the presence of 10−8 M atropine, the concentration-effect curve for the inhibitory action of oxotremorine was shifted to the right in a parallel manner. From the dose-ratio a pA2 value for atropine against oxotremorine of 8.8 was calculated.

  4. 4.

    Hexamethonium (2.8×10−4 M) did not affect the modulating effects of oxotremorine or atropine on acetylcholine output.

  5. 5.

    It is concluded that the guinea-pig ileum myenteric plexus contains inhibitory muscarine receptors whose stimulation by oxotremorine or by liberated endogenous acetylcholine leads to a diminished output of transmitter. Atropine, by blocking these receptors facilitates acetylcholine release. Further, the results suggest that the depression of acetylcholine release per stimulus with increasing frequencies of stimulation is due to the presence of the inhibitory muscarine receptors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ambache, N.: The use and limitations of atropine for pharmacological studies on autonomic effectors. Pharmacol. Rev. 7, 467–494 (1955)

    Google Scholar 

  • Aquilonius, S. M., Lundholm, B., Winbladh, B.: Effects of some anticholinergic drugs on cortical acetylcholine release and motor activity in rats. Eur. J. Pharmacol. 20, 224–230 (1972)

    Google Scholar 

  • Arunlakshana, O., Schild, H. O.: Some quantitative uses of drug antagonists. Br. J. Pharmacol. 14, 48–58 (1959)

    Google Scholar 

  • Bourdois, P. S., Mitchell, J. F., Somogyi, G. T., Szerb, J. C.: The output per stimulus of acetylcholine from cerebral cortical slices in the presence or absence of cholinesterase inhibition. Br. J. Pharmacol. 52, 509–517 (1974)

    Google Scholar 

  • Bülbring, E., Wajda, I.: Biological comparison of local anaesthetics. J. Pharmacol. Exp. Ther. 85, 78–84 (1945)

    Google Scholar 

  • Cohen, J. A., Oosterbaan, R. A.: The active site of acetylcholinesterase and related esterases and its reactivity towards substrates and inhibitors. In: Handbuch der experimentellen Pharmakologie, Vol. XV, pp. 299–373. Berlin-Göttingen-Heidelberg: Springer 1963

    Google Scholar 

  • Cowie, A. L., Kosterlitz, H. W., Watt, A. J.: Mode of action of morphine-like drugs on autonomic neuro-effectors. Nature 220, 1040–1042 (1968)

    Google Scholar 

  • Cox, B., Hecker, S. E.: Investigation of the mechanism of action of oxotremorine on the guinea-pig isolated ileum preparation. Br. J. Pharmacol. 41, 19–25 (1971)

    Google Scholar 

  • Dudar, J. D., Szerb, J. C.: The effect of topically applied atropine on resting and evoked cortical acetylcholine release. J. Physiol. (Lond.) 203, 741–762 (1969)

    Google Scholar 

  • Jones, B. E., Guyenet, P., Chéramy, A., Gauchy, C., Glowinski, J.: The in vivo release of acetylcholine from cat caudate nucleus after pharmacological and surgical manipulations of dopaminergic nigrostriatal neurons. Brain Res. 64, 355–369 (1973)

    Google Scholar 

  • Kilbinger, H.: Modulation by oxotremorine and atropine of acetylcholine release evoked by electrical stimulation of guinea-pig ileum longitudinal muscle strips. Naunyn-Schmiedeberg's Arch. Pharmacol. 287, R8 (1975)

    Google Scholar 

  • Kilbinger, H.: Muscarinic modulation of acetylcholine release from the myenteric plexus of the guinea-pig small intestine. Symp. Cholinergic Mechanism and Psychopharmacol., La Jolla, Calif., March 1977. New York: Plenum Press (in press, 1977)

    Google Scholar 

  • Kilbinger, H., Wagner, P.: Inhibition by oxotremorine of acetylcholine resting release from guinea-pig ileum longitudinal muscle strips. Naunyn-Schmiedeberg's Arch. Pharmacol. 287, 47–60 (1975)

    Google Scholar 

  • Kilbinger, H., Wagner, A., Zerban, R.: Some pharmacological properties of the false cholinergic transmitter acetylpyrrolidinecholine and its precursor pyrrolidinecholine. Naunyn-Schmiedeberg's Arch. Pharmacol. 295, 81–87 (1976)

    Google Scholar 

  • Knoll, J., Vizi, E. S.: Effect of frequency of stimulation on the inhibition by noradrenaline of the acetylcholine output from parasympathetic nerve terminals. Br. J. Pharmacol. 41, 263–272 (1971)

    Google Scholar 

  • Koelle, G. B.: Cytological distributions and physiological functions of cholinesterase. In: Handbuch der experimentellen Pharmakologie. Vol. XV, pp. 187–298. Berlin-Göttingen-Heidelberg: Springer 1963

    Google Scholar 

  • Kosterlitz, H. W., Lees, G. M.: Pharmacological analysis of intrinsic intestinal reflexes. Pharmacol. Rev. 16, 301–339 (1964)

    Google Scholar 

  • MacIntosh, F. C.: Synthesis and storage of acetylcholine in nervous tissue. Can. J. Biochem. Physiol. 41, 2555–2571 (1963)

    Google Scholar 

  • Molenaar, P. C., Polak, R. L.: Stimulation by atropine of acetylcholine release and synthesis in cortical slices from rat brain. Br. J. Pharmacol. 40, 406–417 (1970)

    Google Scholar 

  • Paton, W. D. M., Vizi, E. S.: The inhibitory action of noradrenaline and adrenaline on acetylcholine output by guinea-pig ileum longitudinal muscle strip. Br. J. Pharmacol. 35, 10–28 (1969)

    Google Scholar 

  • Paton, W. D. M., Zar, M. A.: The origin of acetylcholine released from guinea-pig intestine and longitudinal muscle strips. J. Physiol. (Lond.) 194, 13–33 (1968)

    Google Scholar 

  • Paton, W. D. M., Vizi, E. S., Zar, M. A.: The mechanism of acetylcholine release from parasympathetic nerves. J. Physiol. (Lond.) 215, 819–848 (1971)

    Google Scholar 

  • Polak, R. L.: Stimulating action of atropine on the release of acetylcholine by rat cerebral cortex in vitro. Br. J. Pharmacol. 41, 600–606 (1971)

    Google Scholar 

  • Portig, P. J., Vogt, M.: Release into the cerebral ventricles of substances with possible transmitter function in the caudate nucleus. J. Physiol. (Lond.) 204, 687–715 (1969)

    Google Scholar 

  • Silver, A.: The biology of cholinesterases. Amsterdam: North-Holland Publ. Comp. 1974

    Google Scholar 

  • Starke, K.: Regulation of noradrenaline release by presynaptic receptor systems. Rev. Physiol. Biochem. Pharmacol. 77, 1–124 (1977)

    Google Scholar 

  • Szerb, J. C., Somogyi, G. T.: Depression of acetylcholine release from cerebral cortical slices by cholinesterase inhibition and by oxotremorine. Nature New Biol. 241, 121–122 (1973)

    Google Scholar 

  • Vizi, E. S.: Interaction between adrenergic and cholinergic systems: Presynaptic inhibitory effect of noradrenaline on acetylcholine release. J. Neural Transmission. Suppl. XI, 61–78 (1974)

    Google Scholar 

  • Whittaker, V. P., Sheridan, M. N.: The morphology and acetylcholine content of isolated cerebral cortical synaptic vesicles. J. Neurochem. 12, 363–372 (1965)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kilbinger, H. Modulation by oxotremorine and atropine of acetylcholine release evoked by electrical stimulation of the myenteric plexus of the guinea-pig ileum. Naunyn-Schmiedeberg's Arch. Pharmacol. 300, 145–151 (1977). https://doi.org/10.1007/BF00505045

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00505045

Key words

Navigation