Abstract
Localization of NAD+-dependent (type I) 15-hydroxyprostaglandin dehydrogenase (15PGDH) in the rat kidney was examined using an ultramicro assay of the enzyme activity based on the enzymatic cycling method. The enzyme activities during first 3 weeks of age were 30- to 40-fold higher than the adult and rapidly decreased by 4th week. 15PGDH activities measured with either PGE2 or PGF2α as a substrate were five times higher in slices from midcortical or juxtamedullary layers than in slices from the superficial cortex of 3 week-old rat kidney. Little activity was found in inner medulla and papilla. When the enzyme activity was assayed using isolated nephron segments dissected from collagenase treated slices of 3 week-old rat kidneys, the activity was localized only in the proximal convoluted and straight tubules with either PGs (PGE2: 1.75±0.25 in PCT, 7.70±1.19 in PST, and PGF2α: 1.63±0.39, 6.18±1.52 pmoles NADH/mm/40 min). The kinetic analysis for renal 15PGDH of 3 week-old rats revealed thatK m for PGE2 (8.4 μM) was lower than that for PGF2α (22.6 μM) with constant NAD+, whileV max for both was similar. In contrast, bothK m andV max for NAD+ were identical with either PGs. These data suggest that the rate-limiting factor of type I 15PGDH is the concentration of prostaglandins in the kidney rather than the concentration of NAD+. It is thought that the enzyme may be physiologically significant in inactivating the prostaglandins reaching the proxial tubule in order to ensure that the classical prostaglandins (PGE2 and PGF2α) produced in the medullary interstitium and collecting tubules can effectively regulate the distal nephron function.
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Uchida, S., Nonoguchi, H. & Endou, H. Localization and properties of NAD+-dependent 15-hydroxyprostaglandin dehydrogenase activity in the rat kidney. Pflugers Arch. 404, 278–284 (1985). https://doi.org/10.1007/BF00581251
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DOI: https://doi.org/10.1007/BF00581251