Skip to main content
Log in

Diphenylamine-2-carboxylate, a blocker of the Cl-conductive pathway in Cl-transporting epithelia

Pflügers Archiv Aims and scope Submit manuscript

Abstract

The present study examines the effects of diphenylamine-2-carboxylate (DPC) in Cl-transporting epithelia. This substance blocks reversibly the Cl-conductance present under normal circumstances in the basolateral membrane of the thick ascending limb of the loop of Henle (TAL) and in the apical membrane of shark rectal gland tubules (RGT). This leads to a reduction in active NaCl reabsorption (TAL) and NaCl secretion (RGT) respectively, as measured by the equivalent short circuit current. The cells hyperpolarize as the membrane voltage drifts from the control value (some compromise between the chemical potential of Cl and K+) towards the chemical potential of K+. The resistance of the basolateral (TAL) or apical membrane (RGT) increases and this leads to a moderate increase in transepithelial resistance. In addition, the Cl-concentration step induced membrane voltage changes, which can be produced under control conditions, disappear in the presence of the blocker. Finally, experiments in excised membrane patches indicate that this substance inhibits the single current events of individual Cl-channels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Cabantchik ZI, Rothstein A (1972) The nature of the membrane sites controlling anion permeability of human red blood cells as determined by studies with disulfonic stilbene derivatives. J Membr Biol 10:311–330

    Google Scholar 

  2. Cousin JL, Motais R (1982) Inhibition of anion transport in the red blood cell by anionic amphiphilic compounds. I. Determination of the flufenamate-binding site by proteolytic dissection of the band 3 protein. Biochim Biophys Acta 687:147–155

    Google Scholar 

  3. Gögelein H, Greger R (1984) Single channel recordings from basolateral and apical membranes of renal proximal tubules. Pflügers Arch 401:424–426

    Google Scholar 

  4. Greger R (1985) Ion transport mechanisms in the thick ascending limb of Henle's loop of the mammalian nephron. In: American Physiol Soc, Physiological Reviews, Bethesda, Maryland (in press)

  5. Greger R, Schlatter E (1983) Properties of the lumen membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch 396:315–324

    Google Scholar 

  6. Greger R, Schlatter E (1983) Cellular mechanism of the action of loop diuretics on the thick ascending limb of Henle's loop. Klin Wochenschr 61:1019–1027

    Google Scholar 

  7. Greger R, Schlatter E (1984) Mechanism of NaCl secretion in the rectal gland of spiny dogfish (Squalus acanthias). I. Experiments in isolated in vitro perfused rectal gland tubules. Pflügers Arch 402:63–75

    Google Scholar 

  8. Greger R (1984) Ion transport processes in apical membranes of epithelia. In: Wartnock DG, Greger R, Dunham PB, Benjamin MA, Frizzell RA, Field M, Spring KR, Ives HE, Aronson PS, Seifter J (eds) Symposium summary. Fed Proc 43:2473–2487

  9. Greger R, Schlatter E (1984) Mechanism of NaCl secretion in rectal gland tubules of spiny dogfish (Squalus acanthias). II. Effects of inhibitors. Pflügers Arch 402:364–375

    Google Scholar 

  10. Greger R (1981) Cation selectivity of the isolated perfused cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch 390:30–37

    Google Scholar 

  11. Greger R, Schlatter E, Lang F (1983) Evidence for electroneural sodium chloride cotransport in the cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch 396:308–314

    Google Scholar 

  12. Greger R, Schlatter E, Wang F, Forrest JN Jr (1984) Mechanism of NaCl secretion in rectal gland tubules of spiny dogfish (Squalus acanthias). III. Effects of stimulation of secretion by cyclic AMP. Pflügers Arch 402:376–384

    Google Scholar 

  13. Hannaffin J, Kinne-Saffran E, Friedman D, Kinne R (1983) Presence of a sodium-potassium chloride cotransort system in the rectal gland ofSqualus acanthias. J Membr Biol 75:73–83

    Google Scholar 

  14. Hebert SC, Andreoli TE (1984) The effects of antidiuretic hormone on cellular conductive pathways in mouse medullary thick ascending limbs of Henle. II. Determinants of the ADH-mediated increases in transepithelial voltage and in net Cl absorption. J Membr Biol 80:221–233

    Google Scholar 

  15. Hebert SC, Andreoli TE (1984) Control of NaCl transport in the thick ascending limb. Am J Physiol 246:F745-F756

    Google Scholar 

  16. Koenig B, Ricapito S, Kinne R (1983) Chloride transport in the thick ascending limb of Henle's loop: potassium dependence and stoichiometry of the NaCl cotransport system in plasma membranes vesicles. Pflügers Arch 399:173–179

    Google Scholar 

  17. Oberleithner H, Ritter M, Lang G, Guggino W (1983) Anthracene-9-carboxylic acid inhibits renal chloride reabsorption. Pflügers Arch 398:172–174

    Google Scholar 

  18. Palade PT, Barchi RL (1977) On the inhibition of muscle membrane chloride conductance by aromatic carboxylic acids. J Gen Physiol 69:879–896

    Google Scholar 

  19. Sakmann B, Neher E (1983) Single-channel recording. In: Sakmann B, Neher E (eds) Plenum Press. New York London

    Google Scholar 

  20. Schlatter E, Greger R (1984) Mechanism of cAMP-stimulation of active NaCl reabsorption in the isolated perfused medullary thick ascending limb of Henle's loop (mTAL) of mouse nephron. Pflügers Arch 400:R22

    Google Scholar 

  21. Shorofsky SR, Field M, Fozzard HA (1982) The cellular mechanism of active chloride secretion in vertebrate epithelia: studies in intestine and trachea. Philos Trans R Soc Lond [Biol] 299:597–607

    Google Scholar 

  22. Ullrich KJ, Rumrich G, Klöss S (1985) Contraluminal sulfate transport in the proximal tubule of the rat kidney. III. Specificity: disulfonates, di- and tricarboxylates and sulfocarboxylates. Pflügers Arch 404:300–306

    Google Scholar 

  23. Welsh MJ, Smith PL, Frizzell RA (1982) Chloride secretion by canine tracheal epithelium. II. The cellular electrical potential profile. J Membr Biol 70:227–238

    Google Scholar 

  24. Welsh MJ (1984) Anthracene-9-carboxylic acid inhibits an apical membrane chloride conductance in canine tracheal epithelium. J Membr Biol 78:61–71

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Supported by Deutsche Forschungsgemeinschaft, Gr 480/6

Rights and permissions

Reprints and permissions

About this article

Cite this article

Di Stefano, A., Wittner, M., Schlatter, E. et al. Diphenylamine-2-carboxylate, a blocker of the Cl-conductive pathway in Cl-transporting epithelia. Pflugers Arch. 405 (Suppl 1), S95–S100 (1985). https://doi.org/10.1007/BF00581787

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00581787

Key words

Navigation