Summary
The development of cross-resistance to many natural product anticancer drugs, termed multidrug resistance (MDR), is one of the major reasons why cancer chemotherapy ultimately fails. This type of MDR is often associated with over-expression of theMDR1 gene product, P-glycoprotein (Pgp), a multifunctional drug transporter. The expression of MDR in breast tumors is related to their origination from a tissue that constitutively expresses Pgp as well as to the development of resistance during successive courses of chemotherapy. Therefore, understanding the mechanisms that regulate the transcriptional activation ofMDR1 may afford a means of reducing or eliminating MDR. We have found thatMDR1 expression can be modulated by type I cAMP-dependent protein kinase (PKA), opening up the possibility of modulating MDR by selectively down-regulating the activity of PKA-dependent transcription factors which upregulateMDR1 expression. High levels of type I PKA occurs in primary breast carcinomas and patients exhibiting this phenotype show decreased survival. The selective type I cAMP-dependent protein kinase (PKA) inhibitors, 8-Cl-cAMP and Rp8-Cl-cAMP[S] may be particularly useful for downregulating PKA-dependent MDR-associated transcription factors, and we have found these compounds to downregulate transient expression of a reporter gene under the control of severalMDR1 promoter elements. Thus, investigations of this nature should not only lead to a greater understanding of the mechanisms governing the expression of MDR, but also provide a focus for pharmacologic intervention by a new class of inhibitors.
Similar content being viewed by others
References
Beck WT: The cell biology of multiple drug resistance. Biochem Pharmacol 36: 2879–2887, 1987
Gottesman MM, Pastan I: The multidrug transporter, a double-edged sword. J Biol Chem 263: 12163–12166, 1988
Chen C-J, Chin JE, Ueda K, Clark DP, Pastan I, Gottesman MM, Roninson IB: Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells. Cell 47: 381–389, 1986
Gros P, Croop J, Housman DE: Mammalian multidrug resistance gene: complete cDNA sequence indicates strong homology to bacterial transport proteins. Cell 47: 371–380, 1986
Ueda K, Cardarelli C, Gottesman MM, Pastan I: Expression of full-length cDNA for the human ‘MDR1’ gene confers resistance to colchicine, doxorubicin, and vinblastine. Proc Natl Acad Sci USA 84: 3004–3008, 1986
Gerlach JH, Endicott JA, Juranka PF, Henderson G, Sarangi F, Deuchars KL, Ling V: Homology between P-glycoprotein and a bacterial haemolysin transport protein suggests a model for multidrug resistance. Nature 324: 485–489, 1986
Hamada H, Tsuruo T: Purification of the 170- to 180-kilodalton membrane glycoprotein associated with multidrug resistance. J Biol Chem 263: 1454–1458, 1988
Kamimoto Y, Gatmaitan Z, Hsu J, Arias IM: The function of Gp170, the multidrug resistance gene product, in rat liver canalicular membrane vesicles. J Biol Chem 264: 11693–11698, 1989
Naito M, Hamada H, Tsuruo T: ATP/Mg2+-dependent binding of vincristine to the plasma membrane of multidrug-resistant K562 cells. J Biol Chem 263: 11887–11891, 1988
Croop JM, Gros P, Housman DE: Genetics of multidrug resistance. J Clin Invest 81: 1303–1309, 1988
Juranka PF, Zastawny RL, Ling V: P-glycoprotein: multidrug resistance and a superfamily of membrane-associated transport proteins. FASEB J 3: 2583–2592, 1989
Ng WF, Sarangi F, Zastawny RL, Veinot-Drebot L, Ling V: Identification of members of the P-glycoprotein multigene family. Mol Cell Biol 9: 1224–1232, 1989
Chin JE, Soffir R, Noonan KE, Choi K, Roninson IB: Structure and expression of the human MDR (P-glycoprotein) gene family. Mol Cell Biol 9: 3808–3820, 1989
Fojo AT, Whang-Peng J, Gottesman MM, Pastan I: Amplification of DNA sequences in human multidrug-resistant KB carcinoma cells. Proc Natl Acad Sci USA 82: 7661–7665, 1985
Riordan JR, Deuchars K, Kartner N, Alan N, Trent J & Ling V: Amplification of P-glycoprotein genes in multidrug-resistant mammalian cell lines. Nature 316: 817–819, 1985
Roninson IB, Chin JE, Choi K, Gros P, Housman DE, Fojo A, Shen D-W. Gottesman MM, Pastan I: Isolation of human mdr DNA sequences amplified in multidrug-resistant KB carcinoma cells. Proc Natl Acad Sci USA 83: 4538–4542, 1986
Scotto KW, Biedler JL, Melera PW: Amplification and expression of genes associated with multidrug resistance in mammalian cells. Science 232: 751–755, 1986
Fairchild CR, Ivy SP, Kao-Shan C-S, Whang-Peng J, Rosen N, Israel MA, Melera PW, Cowan KH, Goldsmith ME: Isolation of amplified and overexpressed DNA sequences from Adriamycin-resistant human breast cancer cells. Cancer Res 47: 5141–5148, 1987
Fuqua SAW, Moretti-Rojas IM, Schneider SL, McGuire WL: P-Glycoprotein expression in human breast cancer cells. Cancer Res 47: 2103–2106, 1987
Croop JM, Guild BC, Gros P, Housman DE: Genetics of multidrug resistance:relationship of a cloned gene to the complete multidrug phenotype. Cancer Res 47: 5982–5988, 1987
Debenham PG, Kartner N, Siminovitch L, Riordan JR, Ling V: DNA-mediated transfer of multiple drug resistance and plasma membrane glycoprotein expression. Mol Cell Biol 2: 881–889, 1982
Gros P, Neriah YB, Croop JM, Housman DE: Isolation and expression of a complementary DNA that confers multidrug resistance. Nature 323: 728–731, 1986
Shen D-W, Fojo A, Roninson IB, Chin JE, Soffir R, Pastan I, Gottesman MM: Multidrug resistance of DNA-mediated transformants is linked to transfer of the human mdr1 gene. Mol Cell Biol 6: 4039–4045, 1986
Sugimoto Y, Tsuruo T: DNA-mediated transfer and cloning of a human multidrug-resistant gene of Adriamycin-resistant myelogenous leukemia K562. Cancer Res 47: 2620–2625, 1987
Thiebaut F, Tsuruo T, Hamada H, Gottesman MM, Pastan I, Willingham MC: Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues. Proc Natl Acad Sci USA 84: 7735–7738, 1987
Sugawara I, Kataoka I, Morishita Y, Hamada H, Tsuruo T, Itoyama S, Mori S: Tissue distribution of P-glycoprotein encoded by a multidrug-resistant gene as revealed by a monoclonal antibody MRK 16. Cancer Res 48: 1926–1929, 1988
Fojo AT, Ueda K, Slamon DJ, Poplack DG, Gottesman MM, Pastan I: Expression of a multidrug-resistance gene in human tumors and tissues. Proc Natl Acad Sci USA 84: 265–269, 1987
Ueda K, Yamano Y, Kioka N, Kakehi Y, Yoshida O, Gottesman MM, Pastan I, Komano T: Detection of multidrug resistance (MDR1) gene expression in human tumors by a sensitive ribonuclease protection assay. Jpn J Cancer Res 80: 1127–1132, 1989
Noonan KE, Beck C, Holzmayer TA, Chin JE, Wunder JS, Andrulis IL, Gazdar AF, Willman CL, Griffith B, Von Hoff DD, Roninson IB: Quantitative analysis ofMDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction. Proc Natl Acad Sci USA 87: 7160–7164, 1990
Salmon SE, Grogan TM, Miller T, Scheper R, Dalton WS: Prediction of doxorubicin resistancein vitro in myeloma, lymphoma, and breast cancer by P-glycoprotein staining. J Natl Cancer Inst 81: 696–701, 1989
Goldstein LJ, Galski H, Fojo A, Willingham MC, Lai S-L, Gazdar A, Pirker R, Green A, Crist W, Brodeur GM, Lieber M, Cossman J, Gottesman MM, Pastan I: Expression of a multidrug resistance gene in human cancers. J Natl Cancer Inst 81: 116–124, 1989
Verrelle P, Meissonnier F, Fonck Y, Feillel V, Dionet C, Kwiatkowski F, Plagne R, Chassagne J: Clinical relevance of immunohistochemical detection of multidrug resistance P-glycoprotein in breast carcinoma. J Natl Cancer Inst 83: 111–116, 1991
Kohno K, Sato S-I, Takano H, Matsuo K-I, Kuwano M: The direct activation of human multidrug resistance gene (MDR1) by anticancer agents. Biochem Biophys Res Commun 165: 1415–1421, 1989
Chin K-V, Chauhan SS, Pastan I, Gottesman MM: Regulation of mdr RNA levels in response to cytotoxic drugs in rodent cells. Cell Growth Differen. 1: 361–365, 1990
Kato S, Nishimura J, Yufu Y, Ideguchi H, Umemura T, Nawata H: Modulation of expression of multidrug resistance (mdr-1) by adriamycin. FEBS Lett 308: 175–178, 1992
Gekeler V, Frese G, Diddens H, Probst H: Expression of a P-glycoprotein gene is inducible in a multidrug resistant human leukemia cell line. Biochem Biophys Res Commun 155: 754–760, 1988
Ueda K, Pastan I, Gottesman MM: Isolation and sequence of the promoter region of the human multidrug-resistance (P-glycoprotein) gene. J Biol Chem 262: 17432–17436, 1987
Ueda K, Clark DP, Chen C-J, Roninson IB, Gottesman MM, Pastan I: The human multidrug resistance (MDR1) gene. cDNA cloning and transcription initiation. J Biol Chem 262: 505–508, 1987
Chen C-J, Clark D, Ueda Pastan I, Gottesman MM, Roninson IB: Genomic organization of the multidrug resistance (MDR1) gene and origin of P-glycoprotein. J Biol Chem 265: 506–514, 1990
Madden CS, Morrow CS, Nakagawa M, Goldsmith ME, Fairchild CR, Cowan KH: Identification of the 5′ and 3′ sequences involved in the regulation of transcription of the humanMDR1 genein vivo. J Biol Chem 268: 8290–8297, 1993
Fairchild CR, Moscow JA, O'Brien EE, Cowan KH: Multidrug resistance in cells transfected with human genes encoding a variant P-glycoprotein and glutathioneS-transferase-Pi. Mol Pharmacol 37: 801–809, 1990
Zastawny RL, Salvino R, Chen J, Benchimol S, Ling V: The core promoter of the P-glycoprotein gene is sufficient to confer differential responsiveness to wild type and mutant p53. Oncogene 8: 1529–1535, 1993
O'Sheas-Greenfield A, Smale ST: Roles of TATA and Initiator elements in determining the start site location and direction of RNA polymerase II transcription. J Biol Chem 267: 1391–1402, 1992
Cornwell MM: The human multidrug resistance gene: sequences upstream and downstream of the initiation site influence transcription. Cell Growth Differen 1: 607–615, 1990
Goldsmith ME, Madden CS, Morrow CS, Cowan KH: A Y-box consensus sequence is required for basal expression of the human multidrug resistance (MDR1) gene. J Biol Chem 268: 5856–5860, 1993
Cornwell MM, Smith DE: SP1 activates the MDR1 promoter through one of two distinct G-rich regions that modulate promoter activity. J Biol Chem 268: 19505–19511, 1993
Ogura M, Takatori T, Tsuruo T: Purification and characterization of NF-R1 that regulates the expression of the human multidrug resistance (MDR1) gene. Nucl Acids Res 20: 5811–5817, 1992
Dorn A, Durand B, Marfing C, Le Meur M, Benoist C, Mathis D: Conserved major histocompatibility complex class II boxes-X and Y are transcriptional control elements and specifically bind nuclear proteins. Proc Natl Acad Sci USA 84: 6249–6253, 1987
Cornwell MM, Smith DE: A signal transduction pathway for activation of theMDR1 promoter involves the protooncogene c-raf kinase. J Biol Chem 268: 15347–15350, 1993
Tanimura H, Kohno K, Sato S-I, Uchiumi T, Miyazaki M, Kobayashi M, Kuwano M: The human multidrug resistance 1 promoter has an element that responds to serum starvation. Biochem Biophys Res Commun 183: 917–924, 1992
Kohno K, Sato S-I, Ichiumi T, Takano H, Kato S, Kuwano M: Tissue-specific enhancer of the human multidrug-resistance (MDR1) gene. J Biol Chem 265: 19690–19696, 1990
Chin K-V, Ueda K, Pastan I, Gottesman MM: Modulation of activity of the promoter of the humanMDR1 gene by Ras and p53. Science 255: 459–462, 1992
Ishii S, Kadonaga JT, Tjian R, Brady JN, Merlino GT, Pastan I: Binding of the Sp1 transcription factor by the human Harveyras1 proto-oncogene promoter. Science 232: 1410–1413, 1986
Borellini F, Glazer RI: Induction of p53-Sp1 DNA-binding heterocomplexes during GM-CSF-dependent proliferation in human erythroleukemia cell line TF-1. J Biol Chem 268: 7923–7928, 1993
Ackerman SL, Minden AG, Williams GT, Bobonis C, Yeung C-Y: Functional significance of an overlapping consensus binding motif for Sp1 and Zif268 in the murine adenosine deaminase gene promoter. Proc Natl Acad Sci USA 88: 7523–7527, 1991
Schaufele F, West BL, Reudelhuber TL: Overlapping Pit-1 and Sp1 binding sites are both essential to full rat growth hormone gene promoter activity despite mutually exclusive Pit-1 and Sp1 binding. J Biol Chem 265: 17189–17196, 1990
Hariharan N, Perry RP: Functional dissection of a mouse ribosomal protein promoter: significance of the polypyrimidine initiator and an element in the TATA-box region. Proc Natl Acad Sci USA 87: 4509–4513, 1990
Pugh BF, Tjian R: Mechanism of transcriptional activation by Sp1: evidence for coactivators. Cell 61: 1187–1197, 1990
Smale ST, Schmidt MC, Berk AJ, Baltimore D: Transcriptional activation by Sp1 as directed through TATA or initiator: specific requirement for mammalian transcription factor TF IID. Proc Natl Acad Sci USA 87: 4509–4513, 1990
Janson L, Pettersson U: Cooperative interactions between transcription factors Sp1 and OTF-1. Proc Natl Acad Sci USA 87: 4732–4736, 1990
Li J, Knight JD, Jackson SP, Tjian R, Botchan MR: Direct interaction between Sp1 and the BPV enhancer E2 protein mediates synergistic activation of transcription. Cell 65: 493–505, 1991
Lee JS, Galvin KM, Shi Y: Evidence for physical interaction between the zinc-finger transcription factors YY1 and Sp1. Proc Natl Acad Sci USA 90: 6145–6149, 1993
Anderson GM, Freytag SO: Synergistic activation of a human promoterin vivo by transcription factor Sp1. Mol Cell Biol 11: 1935–1943, 1991
Mastrangelo IA, Courey AJ, Wall JS, Jackson SP, Tjian R: DNA looping and Sp1 multimer links: a mechanism for transcriptional synergism and enhancement. Proc Natl Acad Sci USA 88: 5670–5674, 1991
Pascal E, Tjian R: Different activation domains of Sp1 govern formation of multimers and mediate transcriptional synergism. Genes Devel 5: 1646–1656, 1991
Su W, Jackson SP, Tjian R, Echols H: DNA looping between sites for transcriptional activation: self-association of DNA-bound Sp1. Genes Devel 5: 820–826, 1991
Segal R, Berk AJ: Promoter activity and distance constraints of one versus two Sp1 binding sites. J Biol Chem 266: 20406–20411, 1991
Ma L, Krishnamachary N, Perbal B, Center MS: HL-60 cells isolated for resistance to vincristine are defective in 12-O-tetradecanoylphorbol-13-acetate induced differentiation and the formation of a functional AP-1 complex. Oncol Res 4: 291–298, 1992
Borellini F, Aquino A, Josephs SF, Glazer RI: Increased expression and DNA-binding activity of transcription factor Sp1 in doxorubicin-resistant HL-60 leukemia cells. Mol Cell Biol 10: 5541–5547, 1990
Rohlff C, Safa B, Rahman A, Cho-Chung YS, Klecker RW, Glazer RI: Reversal of resistance to Adriamycin by 8-Cl-cyclic AMP in Adriamycin-resistant HL-60 leukemia cells is associated with reduction in type I cyclic AMP-dependent protein kinase and cyclic AMP response element-binding protein DNA-binding activity. Mol Pharmacol 43: 372–379, 1992
Hai T, Curran T: Cross-family dimerization of transcription factors Fos/Jun and ATF/CREB alters DNA binding specificity. Proc Natl Acad Sci USA 88: 3720–3724, 1991
Habener JF: Cyclic AMP response element binding proteins: a cornucopia of transcription factors. Mol Endocrinol 4: 1087–1094, 1990
Merino A, Buckbinder L, Mermelstein FH, Reinberg D: Phosphorylation of cellular proteins regulates their binding to the cAMP response element. J Biol Chem 164: 21266–21276, 1989
Riabowol KT, Fink JS, Gilman MZ, Walsh DA, Goodman RH, Feramisco JR: The catalytic subunit of cAMP-dependent protein kinase induces expression of genes containing cAMP-responsive enhancer elements. Nature 336: 83–86, 1988
Yamamoto KK, Gonzalez GA, Biggs WH III, Montminy MR: Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB. Nature 334: 494–498, 1988
Gonzalez GA, Montminy MR: Cyclic AMP stimulates somatostatin gene transcription by phosphorylation of CREB at serine 133. Cell 59: 675–680, 1989
Auwerx J, Sassone-Corsi P: IP-1: a dominant inhibitor of Fos/Jun whose activity is modulated by phosphorylation. Cell 64: 983–993, 1991
Hagiwara M, Alberts A, Brindle P, Meinkoth J, Feramisco J, Deng T, Karin M, Shenolikar S, Montminy M: Transcriptional attenuation following cAMP induction requires PP-2-mediated dephosphorylation of CREB. Cell 70: 105–113, 1992
Wadzinski BE, Wheat WH, Jaspers S, Peruski LF Jr, Lickteig RL, Johnson GL, Klemm DJ: Nuclear protein phosphatase 2A dephosphorylates protein kinase A-phosphorylated CREB and regulates CREB transcriptional stimulation. Mol Cell Biol 13: 2822–2834, 1993
Imagawa M, Chiu R, Karin M: Transcription factor AP-2 mediates induction by two different signal-transduction pathways: protein kinase C and cAMP. Cell 51: 251–260, 1987
Ahlgren A, Simpson ER, Waterman MR, Lund J: Characterization of the promoter/regulatory region of the bovine CYP11A (P450cc) gene: basal and cAMP-dependent expression. J Biol Chem 265: 3313–3319, 1990
Kagawa C, Waterman MR: cAMP-dependent transcription of the human CYP21B (P-450c21) gene requires a cisregulatory element distinct from the consensus cAMP-regulatory element. J Biol Chem 265: 11299–11305, 1990
Chang C-Y, Huang C, Guo I-C, Tsai HM, Wu D-A, Chung B-C: Transcription of the human ferredoxin gene through a single promoter which contains the 3′,5′-cyclic adenosine monophosphate-responsive sequence and Sp1 binding site. Mol Endocrin 6: 1362–1370, 1992
Jackson SP, MacDonald JJ, Lees-Miller S, Tjian R: GC box binding induces phosphorylation of Sp1 by a DNA-dependent protein kinase. Cell 63: 155–165, 1990
Chin KV, Chaukan SS, Abraham I, Sampson KE, Krolczyk AJ, Wong M, Schimmer B, Pastan I, Gottesman MM: Reduced mRNA levels for the multidrug-resistance genes in cAMP-dependent protein kinase mutant cell lines. J Cell Physiol 152: 87–94, 1992
Hunter T, Karin M: The regulation of transcription by phosphorylation. Cell 70: 375–387, 1992
Yokozaki H, Budillon A, Clair T, Kelley K, Cowan KH, Rohlff C, Glazer RI, Cho-Chung YS: 8-Chloroadenosine 3′,5′-monophosphate as a novel modulator of multidrug resistance. Intl J Oncol 3: 423–430, 1993
Cho-Chung YS: Site-selective cAMP analogs in the arrest of cancer cell growth. In: Glazer RI (ed) Developments in Cancer Chemotherapy. CRC Press, Boca Raton, FL, Vol. II, 1989, pp 77–93
Cho-Chung YS: Role of cyclic AMP receptor proteins in growth, differentiation, and suppression of malignancy: new approaches to therapy. Cancer Res 50: 7093–7100, 1990
Tagliaferi P, Katsaros D, Clair T, Ally S, Tortora G, Neckers L, Rubalcava B, Parandoosh Z, Chang YA, Revankar GR, Crabtree GW, Robins RK, Cho-Chung YS: Synergistic inhibition of growth of breast and colon human cancer cell lines by site-selective cyclic AMP analogues. Cancer Res 48: 1642–1650, 1988
Cho-Chung YS: Differentiation therapy of cancer targeting the RIα regulatory subunit of cAMP-dependent protein kinase (Review). Int J Oncol 3: 141–148, 1993
Rohlff CR, Clair T, Cho-Chung YS: 8-Cl-cAMP induces truncation and down-regulation of the RIα subunit and upregulation of the RIIβ subunit of cAMP-dependent protein kinase leading to type II holoenzyme-dependent growth inhibition and differentiation of HL-60 leukemia cells. J Biol Chem 268: 5774–5782, 1993
Tortora G, Yokozaki H, Pepe S, Clair T, Cho-Chung YS: Differentiation of HL-60 leukemia by type I regulatory subunit antisense oligodeoxynucleotide of cAMP-dependent protein kinase. Proc Natl Acad Sci USA 88: 2011–2015, 1991
Cho-Chung YS, Clair T, Tagliaferri P, Ally S, Katsaros D, Tortora G, Neckers L, Avery TL, Crabtree GW, Robins RK: Site-selective cyclic AMP analogs as new biological tools in growth control differentiation, and proto-oncogene regulation. Cancer Invest 7: 161–177, 1989
Cho-Chung YS, Clair T, Shepheard C: Anticarcinogenic effect of N6,O2-dibutyryl cyclic adenosine 3′,5′-monophosphate on 7,12-dimethylbenz(a)anthracene mammary tumor induction in the rat and its relationship to cyclic adenosine 3′,5′- monophosphate metabolism and protein kinase. Cancer Res 43: 2736–2740, 1983
Cho-Chung YS, Ceresto A, Budillon A, Clair T, Rohlff C: The regulatory subunit of cAMP-dependent protein kinase as a target for cancer diagnosis and therapy. In: Cittadini A (ed) Molecular Oncology and Clinical Applications. Birkhauser Verlag AG, Basel, 1993, pp 267–278
Handschin JC, Eppenberger U: Altered cellular ration of type I and type II cyclic AMP-dependent protein kinase in human mammary tumors. FEBS Lett 106: 301–304, 1979
Miller WR, Jack WW, Chetty U, Elton RA: Tumour cyclic AMP binding proteins: an independent prognostic factor for disease recurrence and survival in breast cancer. Breast Cancer Res Treat 26: 89–94, 1993
Hofmann F, Beavo JA, Krebs EG: Comparison of adenosine 3′,5′-monophosphate-dependent protein kinase from rabbit skeletal and bovine heart muscle. J Biol Chem 250: 7795–7801, 1975
Potter, RL, Taylor SS: The structural domains of cAMP-dependent protein kinase I. J Biol Chem 255: 9706–9712, 1980
Steinberg RA, Cauthron RD, Symcox MM, Shuntoh H: Autoactivation of catalytic (Cα) subunit of cyclic AMP-dependent protein kinase by phosphorylation of threonine 197. Mol Cell Biol 13: 2332–2341, 1993
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Glazer, R.I., Rohlff, C. Transcriptional regulation of multidrug resistance in breast cancer. Breast Cancer Res Tr 31, 263–271 (1994). https://doi.org/10.1007/BF00666159
Issue Date:
DOI: https://doi.org/10.1007/BF00666159