Summary
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1.
Differential regulation, by dexamethasone, of glucocorticoid receptor gene expression was studied in three different neuronal cultures derived from hypothalamus amygdala, and cerebral cortex.
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2.
Cellular glucocorticoid receptor (GR) mRNA concentration was measured by hybridization using a32P-labeled RNA probe complementary to a 2.2-kb fragment of the glucocorticoid receptor mRNA. Changes in the amount of GR mRNA were evaluated in relation to the content ofβ-actin mRNA.
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3.
In cells derived from either hypothalamus or cerebral cortex, we observed a complex pattern of GR mRNA concentrations which were characterized by cyclic variations of GR mRNA content during continuous treatment with dexamethasone for up to 72 hr.
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4.
In contrast to cells derived from the hypothalamus where a persistent 30–40% reduction in GR mRNA levels was seen for up to a least 72 hr, we observed, in cells derived from the cerebral cortex, a sustained increased (1.4-fold) of the GR mRNA at this same time interval.
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Pepin, MC., Beaulieu, S. & Barden, N. Differential regulation by dexamethasone of glucocorticoid receptor messenger RNA concentrations in neuronal cultures derived from fetal rat hypothalamus and cerebral cortex. Cell Mol Neurobiol 10, 227–235 (1990). https://doi.org/10.1007/BF00734576
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DOI: https://doi.org/10.1007/BF00734576