Abstract
The amphiphylic alkyl cation cetyltrimethylammonium inhibits the catalytic activity of soluble and membrane-bound F1 in a noncompetitive fashion. In sonic submitochondrial particles the Dixon plot showed a peculiar pattern with upward deviation at cetyltrimethylammonium concentration higher than 80µM. In membrane-bound F1 the inhibition by cetyltrimethylammonium was potentiated by the F0 inhibitor ologomycin. Cetyltrimethylammonium also inhibited the oligomycin-sensitive proton conductivity in F1-containing particles but was without any effect in F1-depleted particles. Also this inhibitory effect was potentiated by oligomycin. These results indicate functional cooperative interactions between F0 and F1.
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References
Amzel, L. M., and Pedersen, P. L. (1983).Annu. Rev. Biochem. 52, 801–824.
Beechey, R. B., Hubbard, S. A., Linett, P. E., Mitchell, A. d., and Munn, E. A. (1975).Eur. J. Biochem. 148, 533–537.
Foster, D. L., and Fillingame, R. H. (1982).J. Biol. Chem. 257, 2009–2015.
Godinot, C., and Di Pietro, A. (1986).Biochimie 68, 367–374.
Guerrieri, F., Yagi, A., Yagi, T., and Papa, S. (1984).J. Bioenerg. Biomembr. 16, 251–262.
Guerrieri, F., Scarfò, R., Zanotti, F., Che, Y. W., and Papa, S. (1987).FEBS Lett. 213, 67–72.
Houstek, J., Kopecky, J., Zanotti, F., Guerrieri, F., Jirillo, E., Capozza, G., and Papa, S. (1988).Eur. J. Biochem., in press.
Kopecky, J., Guerrieri, F., and Papa, S. (1983).Eur. J. Biochem. 131, 17–24.
Kozlov, I. A., and Skulachev, V. P. (1977).Biochim. Biophys. Acta 463, 1–27.
Lee, C. P., and Ernster, L. (1968).Eur. J. Biochem. 3, 391–400.
Löw, H., and Vallin, I. (1963).Biochim. Biophys. Acta 69, 361–374.
Lowry, O. H., Rosenbrough, N. J., Fan, A. L., and Randall, R. J. (1951).J. Biol. Chem. 193, 265–275.
Matsumo-Yagi, A., and Hatefi, Y. (1984).Biochemistry 23, 3508–3513.
Norling, B., Hundal, T., Sandri, G., Glaser, E., and Ernster, L. (1984). InH +-ATPase ATP Synthase): Structure, Function, Biogenesis. The F 0 F 1 Complex of Coupling Membranes (Papa, S., Altendorf, K., Ernster, L., and Packer, L., eds.), ICSU Press, Miami/Adriatica Editrice, Bari, pp. 303–320.
Pansini, A., Guerrieri, F., and Papa, S. (1978).Eur. J. Biochem. 92, 545–551.
Pansini, A., Guerrieri, F., and Papa, S. (1979). InMembrane Bioenergetics (Lee, C. P., and Ernster, L., eds.), Addison-Wesley, Reading, massachusetts, pp. 423–428.
Papa, S., Tuena de Gomez-Puyou, M., and Gomez-Puyou, A. (1975).Eur. J. Biochem. 55, 1–8.
Papa, S., Altendorf, K., Ernster, L., and Packer, L. (1984). InH +-ATPase (ATP Synthase): Structure, Function, Biogenesis. The F 0 F 1 Complex of Coupling Membranes, ICSU Press, Miami/Adriatica Editrice, Bari, pp. 000–000.
Penefsky, H. S. (1985).J. Biol. Chem. 238, 3762–3769.
Racker, E., and Horstmann, L. L. (1967).J. Biol. Chem. 242, 2547–2551.
Schafër, G., Riegen, E., and Speichert, M. (1974).Eur. J. Biochem. 46, 613–623.
Tommasino, M., and Capaldi, R. A. (1985).Biochemistry 24, 3972–3976.
Tuena de Gomez-Puyou, M., Gomez-Puyou, A., and Salmon, M. (1977a).Biochim. Biophys. Acta 461, 101–108.
Tuena de Gomez-Puyou, M., Gomez-Puyou, A., and Salmon, M. (1977b).Arch. Biochem. Biophys. 173, 326–331.
Vignais, P. V., and Satre, M. (1984).Mol. Cell. Biochem. 60, 33–70.
Yoshida, M., Sone, M., Hirata, H., and Kagawa, Y. (1978).Biochem. Biophys. Res. Commun. 84, 117–122.
Yoshida, M., Poser, J. W., Allison, W., Esch, F. S., and Futai, M. (1981).J. Biol. Chem. 256, 148–153.
Yoshida, M., Allison, W., Esch, F. S., and Futai, M. (1982).J. Biol. Chem. 257, 10033–10037.
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Bârzu, O., Guerrieri, F., Scarfò, R. et al. Effect of cetyltrimethylammonium on ATP hydrolysis and proton translocation in the F0-F1 H+-ATP synthase of mitochondria. J Bioenerg Biomembr 21, 403–414 (1989). https://doi.org/10.1007/BF00762730
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DOI: https://doi.org/10.1007/BF00762730