Summary
Usingl-[3H]dihydroalprenolol ([3H]DHA), a potentβ-adrenergic antagonist, we demonstrated in breast cancer cells the presence ofβ-adrenergic receptors with high affinity (K d 1–9 nM) as shown by Scatchard analyses. Natural and synthetic agonists inhibited the [3H] DHA binding in the following order of potency:l-isoproterenol=l epinephrine > > >l-norepinephrine, identical to the well-established order of potency for these compounds in producingβ-adrenergic responses. We verified that these compounds actually stimulated cAMP production in breast cancer cells. At the present time, the pathophysiological significance ofβ-adrenergic receptors remains unclear. In view of the importance of cAMP in lactose production and in tumor growth mechanisms, it seems to be important to characterize theβ-adrenergic receptors in breast cancer cells in more detail and study their possible involvement in breast tumor growth.
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Abbreviations
- DHA:
-
dihydroalprenolol
- β-AR:
-
β-adrenergic receptor
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Vandewalle, B., Revillion, F. & Lefebvre, J. Functionalβ-adrenergic receptors in breast cancer cells. J Cancer Res Clin Oncol 116, 303–306 (1990). https://doi.org/10.1007/BF01612908
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DOI: https://doi.org/10.1007/BF01612908