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Evidence for a Na+/K+/Cl− cotransport system in basolateral membrane vesicles from the rabbit parotid

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Summary

Sodium (22Na) transport was studied in a basolateral membrane vesicle preparation from rabbit parotid. Sodium uptake was markedly dependent on the presence of both K+ and Cl− in the extravesicular medium, being reduced 5 times when K+ was replaced by a nonphysiologic cation and 10 times when Cl− was replaced by a nonphysiologic anion. Sodium uptake was stimulated by gradients of either K+ or Cl− (relative to nongradient conditions) and could be driven against a sodium concentration gradient by a KCl gradient. No effect of membrane potentials on KCl-dependent sodium flux could be detected, indicating that this is an electroneutral process. A KCl-dependent component of sodium flux could also be demonstrated under equuilibrium exchange conditions, indicating a direct effect of K+ and Cl− on the sodium transport pathway. KCl-dependent sodium uptake exhibited a hyperbolic dependence on sodium concentration consistent with the existence of a single-transport system withK m =3.2mm at 80mm KCl and 23°C. Furosemide inhibited this transporter withK 0.5=2×10−4 m (23°C). When sodium uptake was measured as a function of potassium and chloride concentrations a hyperbolic dependence on [K] (Hill coefficient =1.31±0.07) were observed, consistent with a Na/K/Cl stoichiometry of 1∶1∶2. Taken together these data provide strong evidence for the electroneutral coupling of sodium and KCl movements in this preparation and strongly support the hypothesis that a Na+/K+/Cl− cotransport system thought to be associated with transepithelial chloride and water movements in many exocrine glands is present in the parotid acinar basolateral membrane.

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  1. Clinical Investigations and Patient Care Branch, National Institute of Dental Research, National Institutes of Health, 20892, Bethesda, Maryland

    R. James Turner, Janet N. George & Bruce J. Baum

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  1. R. James Turner
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  2. Janet N. George
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  3. Bruce J. Baum
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Turner, R.J., George, J.N. & Baum, B.J. Evidence for a Na+/K+/Cl− cotransport system in basolateral membrane vesicles from the rabbit parotid. J. Membrain Biol. 94, 143–152 (1986). https://doi.org/10.1007/BF01871194

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  • DOI: https://doi.org/10.1007/BF01871194

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