Abstract
Normal human blood neutrophils were studied for their capacity to synthesize leukotriene B4 (LTB4) and its Ω-oxidized metabolites after phagocytosis of zymosan. Phagocytosis of serum-opsonized particles led to a higher release of LTs than did unopsonized zymosan. The most stricking effect of phagocytosis was observed when neutrophils were primed with granulocyte-macrophage colony-stimulating factor (GM-CSF): opsonization and GM-CSF synergistically increased LTB4 synthesis by neutrophils.
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P. Poubelle, A. D. Beaulieu, M. Nadeau, M. Laviolette and P. Borgeat,The metabolism of arachidonic acid through the lipoxygenase pathways in human phagocytes. InAdvances in inflammation research. vol. 14. (Eds. I. Otterness, R. Capetola and S. Wong) pp. 17–29, Raven Press, New York 1986.
H. E. Claesson, J. A. Lindgren and B. Gustafsson,Opsonized bacteria stimulate leukotriene synthesis in human leukocytes. Biochim. Biophys. Acta836, 361–367 (1985).
O. H. Nielsen, J. Elmgreen, B. S. Thomsen, I. Ahnfelt-Ronne and A. Wiik,Release of leukotriene B 4 and 5-hydroxyeicosatetraenoic acid during phagocytosis of artificial immune complexes by peripheral neutrophils in chronic inflammatory bowel disease. Clin. Exp. Immunol.65, 465–471 (1986).
H. E. Claesson, U. Lundberg and C. Malmsten,Serum-coated zymosan stimulates the synthesis of leukotriene B 4 in human polymorphonuclear leukocytes. Inhibition by cyclic AMP. Biochem. Biophys. Res. Commun.99, 1230–1237 (1981).
P. E. Poubelle, R. de Medicis and P. H. Naccache,Monosodium urate and calcium pyrophosphate crystals differentially activate the excitation-response coupling sequence of human neutrophils. Biochem. Biophys. Res. Commun.149, 649–657 (1987).
B. Odlander, J. A. Lindgren and H. E. Claesson,Effects of immune complexes, zymosan preparations and ionophore A23187 on leukotriene formation in human leukocytes. Prostaglandins34, 603–610 (1987).
J. Fleischmann, D. W. Golde, R. H. Weisbart and J. C. Gasson,Granulocyte-macrophage colony-stimulating factor enhances phagocytosis of bacteria by human neutrophils. Blood68, 708–711 (1986).
C. A. Dahinden, J. Zingg, F. E. Maly and A. L. de Weck,Leukotriene production in human neutrophils primed by recombinant human granulocyte/macrophage colony-stimulating factor and stimulated with the complement component C5a and fMLP as second signals. J. Exp. Med.167, 1281–1295 (1988).
D. J. Williamson, C. G. Begley, M. A. Vadas and D. Metcalf,The detection and initial characterization of colony-stimulating factors in synovial fluid. Clin. Exp. Immunol. 72, 67–73 (1988).
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Poubelle, P.E., Bourgoin, S., Naccache, P.H. et al. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and opsonization synergistically enhance leukotriene B4 (LTB4) synthesis induced by phagocytosis in human neutrophils. Agents and Actions 27, 388–390 (1989). https://doi.org/10.1007/BF01972830
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DOI: https://doi.org/10.1007/BF01972830