Summary
Factor Xa is a serine proteinase which functions principally at coagulation cascades. Factor Xa-like immunoreactivity has been examined in several human organs. Antibodies to the Factor stained macrophages in some tissues examined, including microglia in the brain white matter. They also stained epithelial cells in the nose, bronchus and duodenum. Some brainstem neurons, such as those in the oculomotor nucleus, substantia nigra and pontine nucleus, were also positive for the Factor. As reported by others, these results suggest that factor Xa may have pleiotrophic functions. Furthermore, the prefential localization to epithelium in the nose and bronchus is interesting in view of the previous notion that several viruses targeting the respiratory tract require factor Xa-like cellular proteinases for their replication and spread.
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References
Gajdusek, C., Carbon, S., Ross, R., Nawroth, P. &Stern, D. (1986) Activation of coagulation releases endothelial cell mitogens.J. Cell Biol. 103, 419–28.
Gasic, G. P., Arenas, C. P., Gasic, T. B. &Gasic, G. J. (1992) Coagulation factors X, Xa, and protein S as potent mitogens of cultured aortic smooth muscle cells.Proc. Natl Acad. Sci. USA,89, 2317–20.
Gotoh, B., Ogasawara, T., Toyoda, T., Inocencio, N. M., Hamaguchi, M. &Nagai, Y. (1990) An endopeptidase homologous to the blood clotting factor X as a determinant of viral tropism in chick embryo.EMBO J. 9, 4189–95.
Hoad, R. B. &Geczy, C. L. (1991) Characterisation of monoclonal antibodies to human factor X/Xa. Initial observations with a quantitative ELISA procedures.J. Immunol. Methods 136, 269–78.
Kido, H., Yokogoshi, Y., Sakai, K., Tashiro, M., Kishino, M., Fukutomi, A. &Katunuma, N. (1992) Isolation and characterization of a novel trypsin-like protease found in rat bronchiolar epithelial Clara cells.J. Biol. Chem. 267, 13573–9.
Lindahl, U. &Kolset, S. O. (1982) Studies, with a luminogenic peptide substrate, on blood coagulation factor X/Xa produced by mouse peritoneal macrophages.Biochem. J. 206, 231–7.
Loberg, E. M. &Torvik, A. (1992) Neuronal uptake of plasma proteins in brain contusions. An immunohistochemical study.Acta Neuropathol. 84, 234–7.
McGeer, P. L., Akiyama, H., Kawamata, T., Yamada, T., Walker, D. G. &Ishii, T. (1992) Immunohistochemical localization of beta-amyloid precursor protein sequences in Alzheimer and normal brain tissue by light and electron microscopy.J. Neurosci. Res. 31, 428–42.
Nagai, Y. (1993) Protease-dependent virus tropism and pathogenicity.Trends Microbiol. 1, 81–7.
Ogasawara, T., Gotoh, B., Suzuki, H., Asaka, J., Shimokata, K., Rott, R. &Nagai, Y. (1992) Expression of factor X and its significance for the determination of paramyxovirus tropism in the chick embryo.EMBO J. 11, 467–72.
Østerud, B., Lindahl, U. &Saljelid, R. (1980) Macrophages produce blood coagulation factors.FEBS Lett. 120, 41–3.
Sakai, K., Kawaguchi, Y., Kishino, Y. &Kido, H. (1993) Electron immunohistochemical localization in rat bronchiolar epithelial cells of triptase Clara, which determine the pneumotropism and pathogenicity of Sendai virus and influenza virus.J. Histochem. Cytochem. 41, 89–93.
Shaw, E., Giddings, J. C., Peake, I. R. &Bloom, A. L. (1979) Synthesis of procoagulant factor VIII, factor VIII related antigen and other coagulation factors by the isolated perfused rat liver.B. J. Haematol.,41, 585–96.
Zacharski, L. R., Dunwiddle, C., Nutt, E. M., Hunt, J. &Memoli, V. A. (1991) Cellular localization of activated factor X by Xa specific probes.Thromb. Haemostas. 65, 545–8.
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Yamada, T., Nagai, Y. Immunohistochemical studies of human tissues with antibody to factor Xa. Histochem J 28, 73–77 (1996). https://doi.org/10.1007/BF02331429
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DOI: https://doi.org/10.1007/BF02331429