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Histone deacetylase inhibitors such as sodium butyrate and trichostatin A inhibit vascular endothelial growth factor (VEGF) secretion from human glioblastoma cells

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Abstract

We investigated the effects of histone deacetylase (HDAC) inhibitors such as sodium butyrate (SB) and trichostatin A (TSA) on the expression of vascular endothelial growth factor (VEGF) by human glioblastoma T98G, U251MG, and U87MG cells. The glioblastoma cells secreted three VEGF isoforms, VEGF (189), (165), and (121), although the expression levels of VEGF differed between the cell types. Treatment with either 5mM SB or 100ng/ml TSA reduced VEGF secretion in conditioned media and reduced VEGF mRNA expression. We also studied the expression of VEGF-B,-C, and-D mRNA in human glioblastoma cells and their modulation by HDAC inhibitors. The PCR products of VEGF-B (357 bp), VEGF-C (501 bp), and VEGF-D (484 bp) were amplified in all glioblastoma cells examined. Treatment with SB reduced the expression of VEGF-D mRNA in U251MG cells and the expression of VEGF-B mRNA in U87MG cells. TSA treatment reduced the expression of VEGF-D in U251MG cells. These results suggest that HDAC inhibitors reduce VEGF secretion and modulate the expression of the other VEGF family members, and therefore may inhibit angiogenesis in glioblastoma tissues.

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Authors and Affiliations

  1. Oncology Research Center, Hokuto Hospital, Kisen 7-5, Inada-cho, Obihiro, 080-0833, Hokkaido, Japan

    Hiroki Sawa, Hiromi Murakami & Yoko Ohshima

  2. Department of Neurosurgery, Hokuto Hospital, Obihiro, Hokkaido, Japan

    Masahiro Murakami, Ichiro Yamazaki, Yasuo Tamura, Tatsuo Mima, Akira Satone, Wataru Ide, Ikuo Hashimoto & Hajime Kamada

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  1. Hiroki Sawa
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  2. Hiromi Murakami
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  3. Yoko Ohshima
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  4. Masahiro Murakami
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  5. Ichiro Yamazaki
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  6. Yasuo Tamura
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  7. Tatsuo Mima
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  8. Akira Satone
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  9. Wataru Ide
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  10. Ikuo Hashimoto
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  11. Hajime Kamada
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Correspondence to Hiroki Sawa.

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Sawa, H., Murakami, H., Ohshima, Y. et al. Histone deacetylase inhibitors such as sodium butyrate and trichostatin A inhibit vascular endothelial growth factor (VEGF) secretion from human glioblastoma cells. Brain Tumor Pathol 19, 77–81 (2002). https://doi.org/10.1007/BF02478931

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  • DOI: https://doi.org/10.1007/BF02478931

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