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TRPA1 agonists delay gastric emptying in rats through serotonergic pathways

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Abstract

Our recent study found that TRPA1 is highly expressed in enterochromaffin cells and that stimulation of these cells with TRPA1 agonists enhances 5-hydroxytryptamine (5-HT) secretion in vitro. Here, to demonstrate the 5-HT-releasing effect of TRPA1 agonists in vivo, we examined the effect of TRPA1 agonists on gastric emptying in rats. The results showed that TRPA1 agonists dose-dependently delayed gastric emptying. Further, the effects of TRPA1 agonists on this delay were abolished in rats treated with a TRPA1 antagonist, an inhibitor of tryptophan hydroxylase, or a 5-HT3 receptor antagonist. Taken together, these results indicate that TRPA1 agonists delay in vivo gastric emptying through serotonergic pathways.

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Acknowledgements

We would like to thank T. Koizumi, M. Yamano, Y. Takinami, R. Takezawa, Y. Takemoto, T. Goto, H. Tanaka, H. Okada, H. Kamada, and M. Okada for their excellent technical assistance and advice.

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Authors and Affiliations

  1. Pharmacology Laboratories, Drug Discovery Research, Astellas Pharma, 21 Miyukigaoka, Tsukuba, Ibaraki, Japan

    Hitoshi Doihara, Katsura Nozawa, Eri Kawabata-Shoda, Ryosuke Kojima, Toshihide Yokoyama & Hiroyuki Ito

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  1. Hitoshi Doihara
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  2. Katsura Nozawa
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  3. Eri Kawabata-Shoda
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  4. Ryosuke Kojima
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  5. Toshihide Yokoyama
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  6. Hiroyuki Ito
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Correspondence to Hitoshi Doihara.

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Doihara, H., Nozawa, K., Kawabata-Shoda, E. et al. TRPA1 agonists delay gastric emptying in rats through serotonergic pathways. Naunyn-Schmied Arch Pharmacol 380, 353–357 (2009). https://doi.org/10.1007/s00210-009-0435-7

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  • DOI: https://doi.org/10.1007/s00210-009-0435-7

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