Abstract
Rationale
Interactions among stress, serotonin 1A (5-HT1A) receptors, and the hypothalamic–pituitary–adrenocortical (HPA) system have been proposed to influence the development of depression in humans. The investigation of depression-relevant behaviors and physiological responses to environmental stressors in animal models of depression may provide valuable insight regarding these mechanisms.
Objectives
The purpose of these experiments was to investigate the interactions among central 5-HT1A receptors, endocrine function, and behavior in an animal model of depression, chronic mild stress (CMS).
Methods
The current study examined behavioral responses to a pleasurable stimulus (sucrose), estrous cycle length (in female rats), and plasma hormone levels following systemic administration of a selective 5-HT1A receptor agonist [(+)8-hydroxy-N,N-dipropyl-2-aminotetralin hydrobromide (8-OH-DPAT); 40 μg/kg, s.c.; administered 15 min prior to sacrifice], in male and female rats exposed to 4 weeks of CMS.
Results
Four weeks of CMS produced a reduction in the intake of 1% sucrose (anhedonia), as well as attenuated adrenocorticotropic hormone (ACTH) responses to 8-OH-DPAT in both male and female rats (22 and 18% lower than the control groups, respectively). Corticosterone and oxytocin responses to 8-OH-DPAT were not altered by exposure to CMS. In female rats, CMS induced a lengthening of the estrous cycle by ∼40%.
Conclusions
CMS produces minor HPA disruptions along with behavioral disruptions. Alterations in 5-HT1A receptor function in specific populations of neurons in the central nervous system may be associated with the CMS model. The current findings contribute to our understanding of the relations that stress and neuroendocrine function have to depressive disorders.
Similar content being viewed by others
References
Ahokas A, Kaukoranta J, Wahlbeck K, Aito M (2001) Estrogen deficiency in severe postpartum depression: successful treatment with sublingual physiologic 17beta-estradiol: a preliminary study. J Clin Psychiatry 62:332–336
American Psychiatric Association (1994) Diagnostic and statistical manual of mental disorders, 4th edn. American Psychiatric Association, Washington, DC
Anisimov VN, Baturin DA, Popovich IG, Zabezhinski MA, Manton KG, Semenchenko AV, Yashin AI (2004) Effect of exposure to light-at-night on life span and spontaneous carcinogenesis in female CBA mice. Int J Cancer 111:475–479
Asnis GM, Halbreich U, Ryan ND, Rabinowicz H, Puig-Antich J, Nelson B, Novacenko H, Friedman JH (1987) The relationship of the dexamethasone suppression test (1 mg and 2 mg) to basal plasma cortisol levels in endogenous depression. Psychoneuroendocrinology 12:295–301
Ayensu WK, Pucilowski O, Mason GA, Overstreet DH, Rezvani AH, Janowsky DS (1995) Effects of chronic mild stress on serum complement activity, saccharin preference, and corticosterone levels in Flinders lines of rats. Physiol Behav 57:165–169
Azpiroz A, Fano E, Garmendia L, Arregi A, Cacho R, Beitia G, Brain PF (1999) Effects of chronic mild stress (CMS) and imipramine administration, on spleen mononuclear cell proliferation response, serum corticosterone level and brain norepinephrine content in male mice. Psychoneuroendocrinology 24:345–361
Bagdy G (1996) Role of the hypothalamic paraventricular nucleus in 5-HT1A, 5-HT2A and 5-HT2C receptor-mediated oxytocin, prolactin and ACTH/corticosterone responses. Behav Brain Res 73:277–280
Bagdy G, Makara GB (1994) Hypothalamic paraventricular nucleus lesions differentially affect serotonin-1A (5-HT1A) and 5-HT2 receptor agonist-induced oxytocin, prolactin, and corticosterone responses. Endocrinology 134:1127–1131
Barden N, Reul JM, Holsboer F (1995) Do antidepressants stabilize mood through actions on the hypothalamic–pituitary–adrenocortical system? Trends Neurosci 18:6–11
Bielajew C, Konkle AT, Merali Z (2002) The effects of chronic mild stress on male Sprague–Dawley and Long–Evans rats. I. biochemical and physiological analyses. Behav Brain Res 136:583–592
Boess FG, Martin IL (1994) Molecular biology of 5-HT receptors. Neuropharmacology 33:275–317
Boyer WF, Feighner JP (1989) An overview of fluoxetine, a new serotonin-specific antidepressant. Mt Sinai J Med (NY) 56:136–140
Carrasco GA, Van de Kar LD (2003) Neuroendocrine pharmacology of stress. Eur J Pharmacol 463:235–272
Chaouloff F, Berton O, Mormède P (1999) Serotonin and stress. Neuropsychopharmacology 21(Suppl 2):28S–32S
Cohen LJ, Grothe DR (1992) Major depression: its recognition and treatment. Part 2. Second-generation and newer antidepressants. Am Pharm NS32:44–49
Cvijić G, Janić-Šibalić V, Demajo M, Karakašević A, Petrović VM, Ivanišević-Milovanović OK (1998) The effects of continuous light and darkness on the activity of monoamine oxidase A and B in the hypothalamus, ovaries and uterus of rats. Acta Physiol Hung 85:269–276
D’Souza DN, Zhang Y, Garcia F, Battaglia G, Van de Kar LD (2002) Destruction of serotonergic nerve terminals prevents fluoxetine-induced desensitization of hypothalamic 5-HT(1A) receptors. Psychopharmacology (Berl) 164:392–400
Di Chiara G, Loddo P, Tanda G (1999) Reciprocal changes in prefrontal and limbic dopamine responsiveness to aversive and rewarding stimuli after chronic mild stress: implications for the psychobiology of depression. Biol Psychiatry 46:1624–1633
Dunčko R, Kiss A, Škultétyová I, Rusnák M, Ježová D (2001) Corticotropin-releasing hormone mRNA levels in response to chronic mild stress rise in male but not in female rats while tyrosine hydroxylase mRNA levels decrease in both sexes. Psychoneuroendocrinology 26:77–89
Edgar VA, Cremaschi GA, Sterin-Borda L, Genaro AM (2002) Altered expression of autonomic neurotransmitter receptors and proliferative responses in lymphocytes from a chronic mild stress model of depression: effects of fluoxetine. Brain Behav Immun 16:333–350
Edgar VA, Silberman DM, Cremaschi GA, Zieher LM, Genaro AM (2003) Altered lymphocyte catecholamine reactivity in mice subjected to chronic mild stress. Biochem Pharmacol 65:15–23
Feldman S, Weidenfeld J (1991) Depletion of hypothalamic norepinephrine and serotonin enhances the dexamethasone negative feedback effect on adrenocortical secretion. Psychoneuroendocrinology 16:397–405
Fiske VM (1941) Effect of light on sexual maturation, estrus cycle and pituitary of the rat. Endocrinology 29:187
Fuller RW (1996) Serotonin receptors involved in regulation of pituitary–adrenocortical function in rats. Behav Brain Res 73:215–219
Gittos MW, Papp M (2001) Antidepressant-like action of AGN 2979, a tryptophan hydroxylase activation inhibitor, in a chronic mild stress model of depression in rats. Eur Neuropsychopharmacol 11:351–357
Grippo AJ, Moffitt JA, Johnson AK (2002) Cardiovascular alterations and autonomic imbalance in an experimental model of depression. Am J Physiol Regul Integr Comp Physiol 282:R1333–R1341
Grippo AJ, Beltz TG, Johnson AK (2003a) Behavioral and cardiovascular changes in the chronic mild stress model of depression. Physiol Behav 78:703–710
Grippo AJ, Francis J, Beltz TG, Felder RB, Johnson AK (2003b) Elevated cytokines in the chronic mild stress model of depression. Soc Neurosci Abstr http://sfn.ScholarOne.com/itin2003/main.html
Grippo AJ, Santos CM, Johnson RF, Beltz TG, Martins JB, Felder RB, Johnson AK (2004) Increased susceptibility to ventricular arrhythmias in a rodent model of experimental depression. Am J Physiol Heart Circ Physiol 286:H619–H626
Halbreich U (2003) The etiology, biology, and evolving pathology of premenstrual syndromes. Psychoneuroendocrinology 28(Suppl 3):55–99
Ivanišević-Milovanovićc OK, Demajo M, Karakašević A, Pantić V (1995) The effect of constant light on the concentration of catecholamines of the hypothalamus and adrenal glands, circulatory adrenocorticotropin hormone and progesterone. J Endocrinol Invest 18:378–383
Knobil E (1990) The GnRH pulse generator. Am J Obstet Gynecol 163:1721–1727
Konkle AT, Baker SL, Kentner AC, Barbagallo LS, Merali Z, Bielajew C (2003) Evaluation of the effects of chronic mild stressors on hedonic and physiological responses: sex and strain compared. Brain Res 992:227–238
Kubera M, Maes M, Holan V, Basta-Kaim A, Roman A, Shani J (2001) Prolonged desipramine treatment increases the production of interleukin-10, an anti-inflammatory cytokine, in C57BL/6 mice subjected to the chronic mild stress model of depression. J Affect Disord 63:171–178
Lanfumey L, Pardon M-C, Laaris N, Joubert C, Hanoun N, Hamon M, Cohen-Salmon C (1999) 5-HT1A autoreceptor desensitization by chronic ultramild stress in mice. NeuroReport 10:3369–3374
Li Q, Brownfield MS, Battaglia G, Cabrera TM, Levy AD, Rittenhouse PA, Van de Kar LD (1993a) Long-term treatment with the antidepressants fluoxetine and desipramine potentiates endocrine responses to the serotonin agonist 6-chloro-2-[1-piperazinyl]-pyrazine (MK-212) and (+/−)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane HCL (DOI). J Pharmacol Exp Ther 266:836–844
Li Q, Levy AD, Cabrera TM, Brownfield MS, Battaglia G, Van de Kar LD (1993b) Long-term fluoxetine, but not desipramine, inhibits the ACTH and oxytocin responses to the 5-HT1A agonist, 8-OH-DPAT, in male rats. Brain Res 630:148–156
Li Q, Muma NA, Battaglia G, Van de Kar LD (1997) A desensitization of hypothalamic 5-HT1A receptors by repeated injections of paroxetine: reduction in the levels of G(i) and G(o) proteins and neuroendocrine responses, but not in the density of 5-HT1A receptors. J Pharmacol Exp Ther 282:1581–1590
Long JA, Evans HM (1922) The oestrous cycle in the rat and its associated phenomena. In: Leuschner AO (ed) Memoirs of the University of California. University of California Press, Berkeley, pp 1–148
Lucki I (1998) The spectrum of behaviors influenced by serotonin. Biol Psychiatry 44:151–162
Maes M, Meltzer HY (1995) The serotonin hypothesis of major depression. In: Bloom FE, Kupfer DJ (eds) Psychopharmacology: the fourth generation of progress. Raven Press, New York, pp 933–944
Maric DK, Matsuyama E, Lloyd CW (1965) Gonadotrophin content of pituitaries of rats in constant estrus induced by continuous illumination. Endocrinology 77:529–536
Murison R, Hansen AL (2001) Reliability of the chronic mild stress paradigm: implications for research and animal welfare. Integr Physiol Behav Sci 36:266–274
Murphy DL, Aulakh C, Mazzola-Pomietto P, Briggs NC (1996) Neuroendocrine responses to serotonergic agonists as indices of the functional status of central serotonin neurotransmission in humans: a preliminary comparative analysis of neuroendocrine endpoints versus other endpoint measures. Behav Brain Res 73:209–214
Muscat R, Willner P (1992) Suppression of sucrose drinking by chronic mild unpredictable stress: a methodological analysis. Neurosci Biobehav Rev 16:507–517
Muscat R, Papp M, Willner P (1992) Reversal of stress-induced anhedonia by the atypical antidepressants, fluoxetine and maprotiline. Psychopharmacology (Berl) 109:433–438
Raap DK, Van de Kar LD (1999) Selective serotonin reuptake inhibitors and neuroendocrine function. Life Sci 65:1217–1235
Ressler KJ, Nemeroff CB (2000) Role of serotonergic and noradrenergic systems in the pathophysiology of depression and anxiety disorders. Depress Anxiety 12(Suppl 1):2–19
Silberman DM, Wald M, Genaro AM (2002) Effects of chronic mild stress on lymphocyte proliferative response. Participation of serum thyroid hormones and corticosterone. Int Immunopharmacol 2:487–497
Solberg LC, Horton TH, Turek FW (1999) Circadian rhythms and depression: effects of exercise in an animal model. Am J Physiol, Regul Integr Comp Physiol 276:R152–R161
Vaczek D (2003) Top 200 prescription drugs of 2003. Online. Pharmacy Times, Jamesburg, http://www.pharmacytimes.com/article.cfm?ID=1314
Van de Kar LD, Blair ML (1999) Forebrain pathways mediating stress-induced hormone secretion. Front Neuroendocrinol 20:1–48
Van de Kar LD, Raap DK, Battaglia G, Muma NA, Garcia F, DonCarlos LL (2002) Treatment of cycling female rats with fluoxetine induces desensitization of hypothalamic 5-HT1A receptors with no change in 5-HT2A receptors. Neuropharmacology 43:45–54
Vicentic A, Li Q, Battaglia G, Van de Kar LD (1998) WAY-100635 inhibits 8-OH-DPAT-stimulated oxytocin, ACTH and corticosterone, but not prolactin secretion. Eur J Pharmacol 346:261–266
Weiss JM, Kilts CD (1995) Animal models of depression and schizophrenia. In: Schatzberg AF, Nemeroff CB (eds) The American Psychiatric Press textbook of psychopharmacology. American Psychiatric Press, Washington, DC, pp 81–123
Westlund Tam LW, Parry BL (2003) Does estrogen enhance the antidepressant effects of fluoxetine? J Affect Disord 77:87–92
Willner P (1997) Validity, reliability and utility of the chronic mild stress model of depression: a 10-year review and evaluation. Psychopharmacology (Berl) 134:319–329
Willner P, Lappas S, Cheeta S, Muscat R (1994) Reversal of stress-induced anhedonia by the dopamine receptor agonist, pramipexole. Psychopharmacology (Berl) 115:454–462
Zhang Y, Gray TS, D’Souza DN, Carrasco GA, Damjanoska KJ, Dudas B, Garcia F, Zainelli GM, Sullivan Hanley NR, Battaglia G, Muma NA, Van de Kar LD (2004) Desensitization of 5-HT1A receptors by 5-HT2A receptors in neuroendocrine neurons in vivo. J Pharmacol Exp Ther 310:59–66
Acknowledgements
This research is published in memory of Dr. Louis D. Van de Kar. The authors are grateful for his guidance and friendship, and he is greatly missed. This research was supported by United States Public Health Service grants NS34154 and MH58448. Oxytocin antiserum was donated by Dr. Lanny C. Keil (NASA Ames Research Center, Moffat Field, CA). The authors would like to thank Dr. Lydia DonCarlos and Mr. Brett Peterson for assistance. All experimental procedures comply with current laws of the United States of America.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Grippo, A.J., Sullivan, N.R., Damjanoska, K.J. et al. Chronic mild stress induces behavioral and physiological changes, and may alter serotonin 1A receptor function, in male and cycling female rats. Psychopharmacology 179, 769–780 (2005). https://doi.org/10.1007/s00213-004-2103-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00213-004-2103-4