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Expression of the Gβ5/R7-RGS protein complex in pituitary and pancreatic islet cells

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References

  1. M. Jayaraman, H. Zhou, L. Jia, M.D. Cain, K.J. Blumer, R9AP and R7BP: traffic cops for the RGS7 family in phototransduction and neuronal GPCR signaling. Trends Pharmacol. Sci. 30(1), 17–24 (2009)

    Article  PubMed  CAS  Google Scholar 

  2. G.R. Anderson, E. Posokhova, K.A. Martemyanov, The R7 RGS protein family: multi-subunit regulators of neuronal G protein signaling. Cell Biochem. Biophys. 54(1–3), 33–46 (2009)

    Article  PubMed  CAS  Google Scholar 

  3. C.K. Chen, P. Eversole-Cire, H. Zhang, V. Mancino, Y.J. Chen, W. He, T.G. Wensel, M.I. Simon, Instability of GGL domain-containing RGS proteins in mice lacking the G protein beta-subunit Gbeta5. Proc. Natl. Acad. Sci. USA 100(11), 6604–6609 (2003)

    Article  PubMed  CAS  Google Scholar 

  4. J.H. Zhang, Z.N. Lai, W.F. Simonds, Differential expression of the G protein β5 gene: analysis of mouse brain, peripheral tissues, and cultured cell lines. J. Neurochem. 75, 393–403 (2000)

    Article  PubMed  CAS  Google Scholar 

  5. J.H. Zhang, W.F. Simonds, Copurification of brain G-protein β5 with RGS6 and RGS7. J. Neurosci. 20: RC59, 1–5 (2000)

    Google Scholar 

  6. A.M. Rojkova, G.E. Woodard, T.C. Huang, C.A. Combs, J.H. Zhang, W.F. Simonds, Gγ subunit-selective G protein β5 mutant defines regulators of G protein signaling protein binding requirement for nuclear localization. J. Biol. Chem. 278(14), 12507–12512 (2003)

    Article  PubMed  CAS  Google Scholar 

  7. K. Saeki, M. Zhu, A. Kubosaki, J. Xie, M.S. Lan, A.L. Notkins, Targeted disruption of the protein tyrosine phosphatase-like molecule IA-2 results in alterations in glucose tolerance tests and insulin secretion. Diabetes 51(6), 1842–1850 (2002)

    Article  PubMed  CAS  Google Scholar 

  8. J.H. Zhang, M. Pandey, E.M. Seigneur, L.M. Panicker, L. Koo, O.M. Schwartz, W. Chen, C.K. Chen, W.F. Simonds, Knockout of G protein beta5 impairs brain development and causes multiple neurologic abnormalities in mice. J. Neurochem. 119(3), 544–554 (2011)

    Article  PubMed  CAS  Google Scholar 

  9. J.H. Zhang, V.A. Barr, Y. Mo, A.M. Rojkova, S. Liu, W.F. Simonds, Nuclear localization of Gβ5 and regulator of G protein signalling 7 in neurons and brain. J. Biol. Chem. 276, 10284–10289 (2001)

    Article  PubMed  CAS  Google Scholar 

  10. Q. Wang, K. Levay, T. Chanturiya, G. Dvoriantchikova, K.L. Anderson, S.D. Bianco, C.B. Ueta, R.D. Molano, A. Pileggi, E.V. Gurevich, O. Gavrilova, V.Z. Slepak, Targeted deletion of one or two copies of the G protein {beta} subunit G{beta}5 gene has distinct effects on body weight and behavior in mice. Faseb J. 25, 3949–3957 (2011)

    Google Scholar 

  11. B.A. Posner, A.G. Gilman, B.A. Harris, Regulators of G protein signaling 6 and 7-purification of complexes with Gβ5 and assessment of their effects on G protein-mediated signaling pathways. J. Biol. Chem. 274, 31087–31093 (1999)

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We thank Drs. Dinesh Gautam, Jean-Marc Guettier, and Jürgen Wess (NIDDK) and Dr. Tao Xie (NIAMS) for their expert assistance with pancreatic islet isolation and MIN6 cell culture. We thank Dr. Ching-Kang Jason Chen (Virginia Commonwealth University Medical Center) for the Gnb5 mutant mice. We also thank Drs. Hina Mehta (NIDDK) and Lily Koo (NIAID) for their help. The Intramural Research Program of the NIDDK supported this research.

Conflict of interest

The authors declare that they have no conflict of interest.

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Author notes

  1. Lylia Nini

    Present address: Department of Physiology and Pharmacology, University of Western Ontario, London, ON, N6A 5C1, Canada

  2. Leelamma M. Panicker

    Present address: Department of Microbiology and Immunology, University of Maryland School of Medicine, 660 West Redwood St., Howard Hall, Room 319C, Baltimore, MD, 21201, USA

Authors and Affiliations

  1. Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bldg. 10/Room 8C-101, 10 Center Dr. MSC 1752, Bethesda, MD, 20892-1752, USA

    Lylia Nini, Jian-Hua Zhang, Mritunjay Pandey, Leelamma M. Panicker & William F. Simonds

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  1. Lylia Nini
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  2. Jian-Hua Zhang
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Correspondence to William F. Simonds.

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Nini, L., Zhang, JH., Pandey, M. et al. Expression of the Gβ5/R7-RGS protein complex in pituitary and pancreatic islet cells. Endocrine 42, 214–217 (2012). https://doi.org/10.1007/s12020-012-9611-5

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  • DOI: https://doi.org/10.1007/s12020-012-9611-5

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